新疆南疆驴源H3N8亚型EIV NA基因序列分析

Sequence Analysis of NA Gene of H3N8 EIV of Donkey in Southern Xinjiang

【目的】明确新疆南疆驴是否感染EIV，及EIV NA基因特征。【方法】根据GenBank登录的EIV基因序列，设计合成1对引物，针对新疆南疆地区2个驴屠宰点33份驴肺进行EIV NA基因RT-PCR扩增、电泳分析、胶回收、测序及序列分析。【结果】中国新疆南疆驴源EIV A/donkey/Xinjiang/1/2015（H3N8）株NA基因长1 413 bp，编码470个氨基酸。与国内外H3N8亚型EIV参考毒株核苷酸同源性为752%~996%，与国内外参考毒株氨基酸同源性为80%~100%，与国内毒株、哈萨克斯坦毒株和印度毒株在一个进化分支内。与GenBank数据库中唯一一个驴源毒株A/donkey/Xinjiang/5/2007（H3N8）糖基化位点个数和分布位置完全一致。【结论】新疆南疆存在驴感染EIV，可能为国内疫情的延续或周边国家传入。

[ Objective] In order to clarify whether donkeys were infected with EIV in Southern Xinjiang and the characteristics of NA gene of EIV. [ Method]According to the sequence of NA gene in GenBank,we designed and synthesized 1 pair of primers. The NA gene was obtained by RT - PCR for the suspected cases infected with EIV in Southern Xinjiang. Then the electrophoresis analysis, gel recovery, sequencing and sequence analysis were utilized. [ Result] The results showed that HA gene of A/donkey/Xinjiang/1/2015 （H3NS） strain was 1,413 bp in length, encoding 470 amino acids. The homology of the nucleotide sequence and the amino acid sequence with domestic and foreign reference strains was 75.2% ~ 99.6% % and 80% 100% respectively. The strain with the Chinese strains , Kazakhstan strain India strain in the same evolutionary branching. Amino acids of glycosylation site was completely consistent with A/donkey/Xinjiang/5/2007 （H3NS）, the only one strain from donkey in the GenBank database. [ Conclusion]There is a donkey EIV infection in Southern Xinjiang, which may be a continuation of the domestic epidemic or might be introduced from neighboring countries. This study has certain significance for the disease prevention and control in Southern Xinjiang.