含电穿孔的细胞膜和核膜跨膜电位仿真

Trans-membrane Potential Simulation of Plasma Membrane and Nuclear Membrane with the Consideration of Electroporation

细胞膜和核膜跨膜电位的计算是实现电穿孔效应准确预测的关键所在。基于球形细胞全电路等效模型,引入介电参数阶跃模型来表征细胞膜的电穿孔效应,定量计算了包含细胞膜电穿孔效应的细胞膜和核膜跨膜电位的幅频特性。结果表明:频率大于104 Hz的脉冲电场作用时细胞膜的跨膜电位明显减小,尤其当频率大于108 Hz时,细胞膜的跨膜电位减小7 d B;频率小于106 Hz的脉冲电场作用时核膜的跨膜电位将显著增加,尤其当频率小于102 Hz时,核膜的跨膜电位增加高达19 d B。因此,当脉冲电场作用于生物细胞时,引入电穿孔效应才能准确揭示其跨膜电位的规律。

Calculation on trans-membrane potential （TMP） of plasma membrane and nuclear membrane plays an important role in accurately predicting the electroporation （EP） effects. Based on full-circuit equivalent model of spherical cell and with the consideration of step model of dielectric parameters in which EP effects were characterized, amplitude-frequency characteristics of TMP of plasma membrane and nuclear membrane with EP were calculated quantitatively. The results indicate that TMP of plasma membrane significantly decreases when the frequency of applied pulsed electric fields （PEF） exceeds 104 Hz, TMP will be specifically reduced by 7 dB when PEF exceeds 108 Hz; Meantime, TMP of nuclear membrane significantly increases when the frequency of applied PEF is under 106 Hz, especially, TMP will increase up to 19 dB when frequency of PEF is under 102 Hz. Therefore, mechanism of TMP can be accurately indicated with the consideration of EP effects when PEF are applied to biological cells.