1.84GHz射频电磁辐射对SD大鼠睾丸能量代谢酶的影响及其抗氧化损伤作用

Effects of 1.84 GHz Radiation-frequency Electromagnetic Radiation on Energy Metabolism and Anti-oxidative Damage of Male SD Rat Testis

为了探讨频率为1.84 GHz的射频电磁辐射对SD大鼠睾丸能量代谢酶的影响及其抗氧化损伤作用,将24只成年雄性SD大鼠按体质量随机分成了1组对照组(即假辐照组)和3组辐照组,采用频率为1.84 GHz、比吸收率(SAR)分别为0.2、0.5、1.5 W/kg的射频电磁辐射,连续辐照3周时间,每周辐照5 d时间,每天辐照30 min时间;辐照后立即麻醉处死SD大鼠,取睾丸组织,左侧睾丸组织给予多聚甲醛固定,右侧睾丸组织制备组织匀浆;采用苏木精-伊红(HE)染色后用十字交叉法观察了SD大鼠生精小管直径,采用化学比色法检测了睾丸组织内乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性及丙二醛(MDA)的浓度。研究结果表明:与对照组相比,辐照组SD大鼠睾丸生精小管直径没有明显缩短,辐照组CAT活性和MDA浓度没有明显差异,但辐照组SOD的活性明显降低(概率P<0.05),且SAR为0.5 W/kg和1.5 W/kg的辐照组LDH和SDH活性明显降低(P<0.05)。在此辐照条件下,频率为1.84 GHz的射频电磁辐射,没有对雄性SD大鼠睾丸组织造成明显的氧化损伤,但是降低了SD大鼠睾丸组织内能量代谢酶和部分抗氧化酶的活性。

To investigate the effect of 1.84 GHz radiation-frequency electromagnetic radiation on the energy metabolism and oxidative damage of male SD rat testis, the experimental rats 24 were divided into four groups： the sham exposure group（control group） and three exposure groups, exposure groups were exposed to 1.84 GHz, specific absorption rate（SAR） 0.2, 0.5, 1.5 W/kg for 3 weeks, 5 d/week, 30 min/d. The SD rats were executed after anesthetized and testis were collected after exposure immediately. The left testis was fixed by paraformaldehyde and the right was collected for tissue supematant; Hematoxylin-eosin（HE） staining combined with decussation imaging analysis was carried out to measure the diameter of seminiferous tubules. Chemical colorimetry was used to detect in the activity of LDH, SDH, SOD and CAT, and the MDA contents in testicular tissue. Compared with the control group, the diameter of seminiferous tubules didn＇t have any extremely difference in every exposure group, CAT activity and MDA contents didn＇t change significantly in three exposure groups, SOD activity of rat testicular tissue decreased markedly after exposure in exposure groups（P〈0.05）, and the activity of LDH and SDH decreased significantly after SAR 0.5 W/kg and 1.5 W/kg exposure（P〈0.05）. The result indicates that after present 1.84 GHz radiation, oxidative damage didn＇t induced, and activity of energy metabolism enzymes and some anti-oxidative enzyme decreased in SD rat testicle.