摘要
目的 观察DNA聚合酶-β（DNA pot-β）在乳腺癌中的表达及其对乳腺癌细胞迁移能力的影响.方法 实时定量聚合酶链反应（Real-time PCR）检测75例乳腺癌组织及配对的癌旁组织中DNA pol-β的表达,分析其与淋巴结转移的关系.转染siRNA-DNA pol-β以观察DNA pol-β对乳腺癌细胞迁移能力的影响.结果 同癌旁组织比较,癌组织中DNA pol-β的表达显著升高（2.80 ±2.27比3.49±2.56,P＜0.01）,淋巴结转移与DNA pol-β的表达呈正相关（χ^2=5.580,P＜0.05）.同空白对照组（23.0±4.0）和阴性对照组（20.1±3.8）比较,siRNA-DNA pol-β转染组（10.1±2.5） MCF-7细胞的迁移能力显著下降（P＜0.05）.结论 DNA pol-β在乳腺癌中表达上调,可能是乳腺癌细胞转移的机制之一。
Objective To investigate the expression of DNA polymerase-β （DNA pol-β） in breast cancer and its effect on migration of breast cancer cells.Methods The mRNA level of DNA pol-β in breast cancer tissues and paired paracancerous tissues were detected by real-time quantitative polymerase chain reaction （Real-time PCR） and the relationship between DNA pol-β expression and lymph node metastasis in patients with breast cancer were analyzed.The effect of DNA pol-β on migration of breast cancer ceils was investigated.Results The expression of DNA pol-β was up-regulated in breast cancer compared with adjacent breast tissues （2.80 ± 2.27 vs.3.49 ± 2.56,P 〈 0.01）.The increased expression of DNA pol-β in breast cancer tissues was positively associated with lymph node metastasis （χ^2 =5.580,P 〈0.05）.MCF-7 cells transfected with siRNA-DNA pol-β （10.1 ±2.5） showed decreased migration compared with control group （23.0 ± 4.0） and negative siRNA group （20.1 ±.3.8） （P 〈 0.05）.Conclusion The up-regulation of DNA pol-β in breast cancer tissue is a possible mechanism responsible for the metastasis of breast cancer cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2016年第9期2135-2137,共3页
Chinese Journal of Experimental Surgery
基金
河南省基础与前沿技术研究计划（142300410207）
