肌动蛋白纤维相关蛋白1-反义RNA1在胃癌中异常表达的意义及相关功能被引量：3

The expression and function of actin filament-associated protein 1-antisense RNA 1 in gastric cancer

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摘要目的探讨长链非编码RNA（lncRNA）肌动蛋白纤维相关蛋白1-反义RNA1（AFAP1-AS1）在胃癌中的表达及其临床意义,并在胃癌细胞株中验证其细胞学功能.方法采用实时荧光定量聚合酶链反应（RT-qPCR）检测我院60例胃癌患者的癌及对应癌旁组织中AFAP1-AS1的水平,分析AFAP1-AS1的表达与临床病理特征的关系;进一步使用RT-qPCR检测5个不同的胃癌细胞株中AFAP1-AS1的表达水平.对于高表达AFAP1-AS1的细胞株使用慢病毒载体下调基础表达.进而检测下调AFAP1-AS1后对细胞增殖、凋亡和侵袭能力的影响.结果胃癌组织中AFAP1-AS1的相对表达量△Ct水平显著高于癌旁组织水平（6.349±1.966比3.065±1.015,P＜0.01）,且其表达与组织学分级、TNM分期、淋巴结转移水平有关（P＜0.05）;胃癌细胞株MGC-803及SGC-7901高表达（2.239±0.020及2.488±0.021）,有效干扰AFAP1-AS1的表达后可以显著抑制MGC-803及SGC-7901细胞的增殖、侵袭能力并促进凋亡（P＜0.01）.结论 AFAP1-AS1在胃癌组织中高表达.沉默AFAP1-AS1后可以显著抑制胃癌细胞的增殖、侵袭能力并促进凋亡。 Objective To explore the expression and clinical significance of long non-coding RNA （IncRNA） actin filament-associated protein 1-antisense RNA 1 （AFAP1-AS1） in gastric cancer （GC）,and to verify its function in GC cells.Methods The levels of AFAP1-AS1 were detected in 60 couples of GC specimens,corresponding adjacent tissues and in 5 gastric cancer cell lines using the real-time quantitative polymerase chain reaction （RT-qPCR）.The relationship between the AFAP1-AS1 and clinicopathological parameters was analyzed.Cells （MGC-803 and SGC-7901） with highly expressed AFAP1-AS1 were down-regulated lentiviral short hairpin RNA （shRNA） directed against AFAP1-AS1.The effects of the AFAP1-AS1 knockdown on cell proliferation,invasion and apoptosis were performed by the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyhetrazolium bromide （MTT）method,flow cytometry with propidium iodide （PI）/annexin V double staining and Transwell.Results The ACt level of AFAP1-AS1 expressed in tumor tissue was significantly higher than that in paracarcinoma tissues （6.349 ± 1.966 vs.3.065 ± 1.015,P 〈0.01）,and related with TNM staging,lymph node metastasis,and tumor differentiation （P 〈 0.05）,respectively.AFAP1-AS1 was highly expressed in MGC-803 and SGC-7901 gastric cancer cell lines （2.239 ± 0.020,and 2.488 ± 0.021）.MTT assay revealed the absorbance values of AFAP1-AS1 knockdown group were lower than in the control group at 48 and 72 h（P 〈0.01）.Moreover,there was higher apoptosis rate but less transmembrance cell number.Conclusion The expression of AFAP1-AS1 was higher in both GC tissues and cells,respectively.Knockdown of AFAP1-AS1 may inhibit the cell proliferation and invasion but induce the apoptosis of GC cells.

作者周立生邓标裘正军

机构地区上海市第一人民医院普外科

出处《中华实验外科杂志》 CAS CSCD 北大核心 2016年第9期2206-2209,共4页 Chinese Journal of Experimental Surgery

关键词胃癌肌动蛋白纤维相关蛋白1-反义RNA1 细胞功能 Gastric cancer Actin filament-associated protein 1 - antisense RNA 1 Cell function

分类号 R735.2 [医药卫生—肿瘤]

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