亚砷酸钠对雄性大鼠肺氧化损伤作用研究

Research on the oxidation damage of sodium arsenite on rat lung

目的探讨亚砷酸钠(NaAsO_2)经饮水染毒对大鼠肺氧化损伤作用。方法无特定病原体级健康成年雄性SD大鼠,随机分为对照组和低、中、高剂量组,每组8只。3个剂量组大鼠自由饮用质量浓度分别为10.00、100.00和1 000.00μg/L的NaAsO_2溶液,对照组大鼠自由饮用超纯水。染毒28 d后处死大鼠,测定支气管肺泡灌洗液(BALF)中白细胞总数和细胞死亡率,以酶联免疫吸附实验测定BALF中活性氧(ROS)、还原型辅酶Ⅱ(NADPH)、总抗氧化能力和一氧化氮(NO)水平。结果中剂量组大鼠BALF中白细胞总数分别高于对照组和高剂量组(P<0.05);高剂量组大鼠BALF中细胞死亡率分别高于对照组、低剂量组和中剂量组(P<0.01)。低、中和高剂量组大鼠BALF中ROS水平分别高于对照组(P<0.01);中、高剂量组大鼠BALF中NADPH水平分别高于对照组和低剂量组(P<0.05);中和高剂量组大鼠BALF中总抗氧化能力分别低于对照组(P<0.01),但NO水平均高于对照组(P<0.05)。结论砷进入大鼠体内后可能是通过激活氧化酶NADPH使ROS高表达,进而使NO增多,造成组织氧化应激损伤。

Objective To explore the oxidation damage of sodium arsenite（ NaAsO2） drinking exposure on rat lung.Methods Thirty-two specific pathogen free healthy adult male SD rats were randomly divided into 4 groups： control group,low-,medium- and high-dose groups,with 8 rats in each group. The 3 arsenic exposure groups were intoxicated by NaAsO2 with mass concentrations of 10. 00,100. 00 and 1 000. 00 μg / L in drinking water,respectively,while the control group was given ultrapure water for free drinking. All the rats were sacrificed after 28 days. The total number of white blood cells and the cell death rate in bronchoalveolar lavage fluid（ BALF） were measured. The level of reactive oxygen species（ ROS）,nicotinamide adenine dinucleotide phosphate（ NADPH）,the antioxidant ability and the nitric oxide（ NO） level in BALF were measured by the enzyme-linked immunosorbent assay. Results The total number of white blood cells in BALF in medium-dose group was higher than those of the control group and high-dose group（ P〈0. 05）.The cell death rate of BALF in high-dose group was higher than those of the other groups（ P〈0. 01）. The ROS levels in BALF of rat lung in 3 exposure groups were higher than that of the control group（ P〈0. 01）. The NADPH levels in medium- and high-dose groups were higher than those of the control group and low-dose group（ P〈0. 05）. The total antioxidant ability in medium- and high-dose groups were lower than that of the control group（ P〈0. 01）. The NO levels in medium- and high-dose groups were higher than that of the control group（ P〈0. 05）. Conclusion The NaAsO2 exposure in rats could lead to high expression of oxidase ROS by activating NADPH followed by increasing NO level,resulting in imbalanced organism oxidation and anti-oxidation system and causing oxidative stress injury in tissues.