毛茛科植物铁棒锤与工布乌头的对比研究

Contrastive Study of Ranunculaceae Aconitum Pendulum Busch and Aconitum Kongboense Lauener

目的:对铁棒锤和工布乌头进行对比,以证明两者是否存在差异,是否需要区别用药。方法:进行两种药材的显微特征、薄层色谱的比较,采用HPLC法测定铁棒锤及工布乌头中乌头碱的含量。结果:两种药材在显微特征上存在一定的差异。以正己烷:乙酸乙酯:二乙胺(10∶6∶0.8)为展开剂,以乌头碱为对照品,结果 TLC图表明两者成分有明显区别。含量测定结果表明,药材铁棒锤的乌头碱含量为0.71 mg·g-1,而工布乌头中乌头碱的含量仅为0.03 mg·g-1。结论:经过显微、TCL、HPLC的对比研究表明,两种药材有较大差异,在用法上应注意区分,不能将工布乌头视作铁棒锤进行用药。

Objective： To identify Aconitum pendulum Busch. and Avonitum kongboense Lauoner. Methods： Aconitum pendulum Busch. and Aconitum kongboense Lauener were identified by microscopical identification and TLC, And the contents of aconitine in the two herbs were determined by HPLC. Results： Microscopical identification showed they were different from each other, and aconitine was used as the reference component. Using the solution consisting of hexane ： ethyl acetate ： diethylamine （ 10 ： 6 ： 0. 8） as the developing solvent,the compositions in the two herbs were different in TLC. The content of aconitine in Avanitum pendulum Busch was 0.71mg·g^-1,while that in Aconitum kongboense Lauener was only 0.03mg·g^-1.Conclusion：Aconitum pendulum Busch,and Aconitum kongboense Lauener Identified by microcopical identification,TLC and HPLC,SHOW NOTABLE differeces between them,and Aconiturn kongboense Lauener should not be used as Aconitum pendulum Busch.