赤雹根总皂苷提取优化工艺考察

Optimization of Extraction Technology for the Total Saponins from the Root of Thladiantha Dubia Bunge

目的:优选赤雹根总皂苷的提取工艺。方法:以赤雹根总皂苷的溶出量为评价指标,以提取次数、提取时间、固液比为考察因素,采用正交试验法优选出赤雹根总皂苷的最佳提取工艺。结果:最佳提取工艺为70%乙醇回流提取2次,每次1.5 h,固液比为1∶6。结论:此工艺操作简便,重复性好,稳定可行。

Objective： To optimize the extraction process for the total saponins from the root of Thladiantha dubia Bunge. Methods： The extraction technology was optimized by orthogonal experiment with the dissolution content of total saponins as the index and the extraction times, extraction duration and ratio of solid to liquid as the influencing factors. Results： The optimum extraction conditions for the total saponins from the root of Thladiantha dubia Bunge were as follows： the reflux extraction was conducted twice（ 1.5 h per time） with 70% ethanol as the solvent, and the ratio of solid to liquid was 1：6. Conclusion： The optimum extraction technology is simple, reproducible and stable.