HPLC法测定畲药十二时辰中活性成分齐墩果酸的含量

Determination of Active Ingredient Oleanolic Acid in She Medicine Clematis Florida var. Plena by HPLC

目的:建立畲药十二时辰中齐墩果酸含量测定的方法。方法:采用高效液相色谱法,选用Lanbo-Kromasil-C-18色谱柱;以乙腈-水(86∶14)为流动相;检测波长205 nm,流速1.0 ml·min^(-1),柱温为25℃。结果:齐墩果酸在进样量0.52~15.60μg(r=0.999 9)范围内呈良好的线性关系。平均加样回收率为92.13%,RSD=3.0%(n=9)。结论:本法简便、快速、准确、重复性好,可作为畲药十二时辰质量控制方法。

Objective： To establish a method for the determination of oleanolic acid in She medicine Clematis florida vat. plena. Methods： The HPLC method was carried on a Lanbo-Kromasil C18 chromatography column using acetonitrile-water （86： 14） as the mobile phase. The detection wavelength was 205 nm, the flow rate was 1.0 ml·min^-1, and the column temperature was 25℃. Results： The calibration curve of oleanolic acid was linear within the range of 0.52-15. 60μg （r = 0.999 9）. The average recovery of oleanolic acid was 92.13% （RSD = 3.0%, n = 9）. Conclusion ： The method is simple, rapid, accurate and reproducible, which can be used as an ideal method for the quality control of She medicine Clematis florida var. plena..