摘要
目的：建立HPLC法同时测定不同产地荔枝草中齐墩果酸和熊果酸的含量。方法：采用Agilent-C18色谱柱（250mm×4．6mm，5μm），流动相为甲醇-水-冰醋酸-三乙胺（90：10：0．03：0．06），检测波长：210nm，流速：1．0ml·min^-1，柱温：25℃。结果：齐墩果酸在7．50-75．00μg·ml^-1、熊果酸在5．00～50．00μg·ml^-1浓度范围内线性关系良好，r均为0．9997。齐墩果酸和熊果酸的平均加样回收率分别为100．15％（RSD=1．14％，n=6）、99．40％（RSD=1．67％，n=6）。结论：该方法简便、准确，适用于荔枝草中齐墩果酸和熊果酸的含量测定。
Objective： To develop an HPLC method for the simultaneous determination of oleanic acid and ursolic acid in Salvia plebeia R. Br. from different origins. Methods： The column was Agilent-C18 （250mm×4.6mm,5μm） with the temperature of 25℃, the mobile phase was methanol-water-glacial acetic acid-triethylamine （90 ： 10：0.03：0.06） at the flow rate of 1.0ml·min^-1, and the detection wavelength was 210 nm. Results： The calibration curves were linear within the range of 7.50-75.00 μg·ml^-1 for oleanic acid（ r = 0.999 7 ） and 5.00-50.00μg·ml^-1 for ursolic acid（ r = 0.999 7 ）. The average recovery of oleanic acid and ursolic acid was 100.15% （ RSD = 1.14% , n = 6 ） and 99.40% （ RSD = 1.67% , n = 6 ） , respectively. ConclusiOn： The method is simple and accurate, which is suitable for the determination of oleanic acid and ursolic acid in Salvia plebeia R. Br. from different origins.
出处
《中国药师》
CAS
2016年第9期1776-1777,1780,共3页
China Pharmacist
