不同时期外源6-BA处理对甘草有效成分的影响研究

Effect of Chemical Constituents from Glycyrrhiza uralensis Induced by Exogenous 6-Benzylaminopurine in Different Growth Periods

目的:研究外源细胞分裂素6-苄氨基嘌呤(6-BA)处理对甘草中主要药用成分的影响,探究甘草中各化学成分之间的调控网络。方法:以两年生甘草Glycyrrhiza uralensis Fisch.植株为试验材料,分别于甘草生长期的两个不同阶段6月、7月喷施4种不同浓度(15、25、50、100 mg/L)的6-BA,利用HPLC检测7种化学成分(甘草酸、甘草苷、异甘草苷、甘草素、异甘草素、芹糖基甘草苷和芹糖基异甘草苷)的含量,分析外源刺激后甘草中7种成分含量的动态变化。结果:(1)6月份6-BA处理各组,7种成分含量的提高幅度最大值均远大于7月份处理组,其中,6月份100 mg/L的6-BA处理3个月后,其7种成分提高幅度分别比对照高出59.34%、71.14%、57.31%、16.36%、30.17%、80.26%、91.90%;(2)不同浓度处理组影响最大的成分为甘草酸;(3)6-BA处理有抵御自然生长条件下甘草中黄酮类含量降低的作用;(4)6-BA处理虽然对甘草中的化学成分含量有一定影响,但并不改变其化学成分间组成变化。结论:外源6-BA处理可以同时提高甘草中甘草酸含量以及黄酮类物质的积累。

Objective： To study the effect of chemical constituents from Glycyrrhiza uralensis induced by exogenous 6-benzylaminopurine（ 6-BA） in different growth periods,aim to explore the regulatory network of Glycyrrhiza uralensis. Methods： The transplants of two-year-old Glycyrrhiza uralensis were subjected to four concentrations of 6-BA（ 15,20,50,100 mg / L） at June and July. The content of seven kinds of chemical constituents including glycyrrhizic acid,liquiritin,isoliquiritin,liquiritigenin,isoliquiritigenin,liquiritin apioside,isoliquiritin apioside were determined by HPLC. Then long-term dynamic changes of the content of seven chemical compositions were analyzed. Results： In June,the content of seven compositions increased remarkably after 6-BA stimulating than that in July. Seven kinds of chemical constituents increased remarkably after 100 mg / L 6-BA stimulating for 3 months,and the increase rates were59. 34%,71. 14%,57. 31%,16. 36%,30. 17%,80. 26%,91. 90%,respectively. The greatest impact was glycyrrhizic acid with different concentration stimulating. 6-BA stress had a protection ability against the decrease of flavonoids in licorice under natural growth condition. The treatment of 6-BA had effect to the content of Glycyrrhiza uralensis,but hardly altered the ratio of their chemical composition. Conclusion： A regulatory network is existed among medicinal ingredients of Glycyrrhiza uralensis. Glycyrrhizic acid and flavonoids accumulation of Glycyrrhiza uralensis can be affected by exogenous 6-BA stimulation in certain case.