双花堇菜乙酸乙酯部位化学成分研究

Chemical Constituents of Ethyl Acetate Extract from Viola biflora

目的：研究双花堇菜乙酸乙酯部位的化学成分。方法：采用硅胶、制备薄层色谱和Sephadex LH-20凝胶色谱法进行分离纯化,通过测定理化常数和各种波谱技术鉴定化合物结构。结果：从双花堇菜乙酸乙酯部位分离鉴定了12个化合物,分别为：金色酰胺醇酯（1）、solalyratin B（2）、秦皮乙素（3）、莨菪亭（4）、羽扇豆醇（5）、132S-羟基脱镁叶绿素a（6）、紫罗兰醇（7）、邻苯二甲酸二丁酯（8）、（-）-二氢催吐萝夫叶醇（9）、蚱蜢酮（10）、crassifol（11）、β-谷甾醇（12）。结论：所有化合物均为首次从该植物中分离得到。其中,化合物2、7、9~11首次从堇菜属植物中分离得到。

Objective： To isolate the chemical constituents of ethyl acetate extract from Viola biflora. Methods： Isolation and purification were carried out on repeated silica gel column chromatography,PTLC,and Sephadex LH-20. The structures of these compounds were elucidated by physico-chemical properties and spectral analyses. Results： Twelve compounds were isolated from Viola biflora,which identified as aurantiamide acetate（ 1）,solalyratin B（ 2）,esculetin（ 3）,scopoletin（ 4）,lupeol（ 5）,132S-hydroxypheophytin a（ 6）,vomifoliol（ 7）,dibutyl phthalate（ 8）,（-）-dihydrovomifoliol（ 9）,grasshopper ketone（ 10）,crassifol（ 11） and β-sitosterol（ 12）. Conclusion：All the compounds are isolated from Viola biflora for the first time. Compounds 2,7,9 ~ 11 are isolated from Viola genus for the first time.