基于指纹图谱结合多元统计分析的天麻总苷差异指标的研究

Study on the Different Indexes of Gastrodia elata Total Glycosides Based on Fingerprint Combined with Multivariate Statistical Analysis

目的:建立10批不同产地天麻中天麻总苷的HPLC指纹图谱,并对建立的指纹图谱信息进行多元统计分析,筛选出可能影响天麻总苷差异的指标性成分。方法:建立天麻总苷的HPLC指纹图谱,运用指纹图谱相似度评价软件对指纹图谱数据进行处理,运用SPSS 19.0进行系统聚类分析和主成分分析,根据主成分分析结果再进行聚类分析,筛选出可能影响天麻总苷差异的指标性成分。结果:天麻总苷HPLC指纹图谱共标记了12个共有峰,根据聚类分析结果,可将10个产地天麻聚为4类,导致差异的原因可能与地理位置和生长环境有一定关系。由PCA分析结果可知,筛选出6个天麻总苷差异成分,将这6个差异成分再进行聚类分析与之前结果基本一致,初步确定了天麻差异的指标性成分。结论:该实验建立了天麻总苷的HPLC指纹图谱,通过多元统计分析,筛选出可能影响天麻总苷差异的指标性成分,为天麻药材的全面质量控制奠定基础,为天麻总苷差异成分与药效关联研究提供参考。

Objective： To establish HPLC fingerprint of Gastrodia elata total glycosides components and carry on multivariate statistical analysis,to screen the indexes compositions that may influence the difference between total glycosides from ten batches. Methods：Established HPLC fingerprint of Gastrodia elata total glycosides components from ten batches. The fingerprint similarity evaluation software was used to process the fingerprint data,SPSS 19. 0 was used to carry out system cluster analysis and principal component analysis（ PCA）. According to the PCA results,another system cluster analysis was carried out to screen the possible indexes compositions that influence the difference between total Gastrodia elata glycosides. Results： Twelve common peaks were remarked,according to the result of clustering analysis,ten batches rhizoma gastrodiae could be divided into four types,and the reasons of the differences may have a certain relationship with geographical location and growth environment. The PCA analysis results showed that,there were 6 different compositions and the cluster analysis results were the same as before,and the different indexes compositions were determined. Conclusion：HPLC fingerprint of Gastrodia elata total glycosides components is established,through multivariate statistical analysis,the indexes compositions that may influence the differences of total gastrodia glycosides are screen,which lay a foundation for the comprehensive quality control,and provide a reference for the study between different compositions and the efficacy.