穿山龙多糖脱蛋白工艺研究

Study on Deproteinization of Polysaccharides from Dioscorea nipponica

目的:筛选穿山龙多糖脱蛋白的最优工艺。方法:以穿山龙多糖保留率和蛋白脱除率为考察指标,研究4种脱蛋白方法对穿山龙多糖脱蛋白的影响。结果:确定木瓜蛋白酶法结合Sevage法为最佳工艺,工艺条件为:酶解温度55℃,木瓜蛋白酶用量2.0%,酶解时间122 min,1次Sevage;在此条件下,穿山龙多糖蛋白脱除率为91.24%,多糖保留率为80.12%。结论:木瓜蛋白酶结合Sevage法是一种良好的穿山龙多糖脱蛋白方法。

Objective： To screen the optimum method for deproteination of Dioscorea nipponica polysaccharides. Methods： The ratio of protein removing and polysaccharides remaining were used as indicator,four deproteination methods were evaluated. Results： PapainSevage method was the best one,in which the deproteination rate was 91. 24%,and 80. 12% of polysaccharide was remained. The optimum conditions for deproteinization were as follows,hydrolysing the substrates with 2. 0% papain（ p H 7. 0） at 55 ℃ for 122 min,and one times with Sevage method. Conclusion： The papain-Sevage is the best deproteination method for purifying Dioscorea nipponica polysaccharides.