摘要
目的:筛选穿山龙多糖脱蛋白的最优工艺。方法:以穿山龙多糖保留率和蛋白脱除率为考察指标,研究4种脱蛋白方法对穿山龙多糖脱蛋白的影响。结果:确定木瓜蛋白酶法结合Sevage法为最佳工艺,工艺条件为:酶解温度55℃,木瓜蛋白酶用量2.0%,酶解时间122 min,1次Sevage;在此条件下,穿山龙多糖蛋白脱除率为91.24%,多糖保留率为80.12%。结论:木瓜蛋白酶结合Sevage法是一种良好的穿山龙多糖脱蛋白方法。
Objective: To screen the optimum method for deproteination of Dioscorea nipponica polysaccharides. Methods: The ratio of protein removing and polysaccharides remaining were used as indicator,four deproteination methods were evaluated. Results: PapainSevage method was the best one,in which the deproteination rate was 91. 24%,and 80. 12% of polysaccharide was remained. The optimum conditions for deproteinization were as follows,hydrolysing the substrates with 2. 0% papain( p H 7. 0) at 55 ℃ for 122 min,and one times with Sevage method. Conclusion: The papain-Sevage is the best deproteination method for purifying Dioscorea nipponica polysaccharides.
出处
《中药材》
CAS
CSCD
北大核心
2016年第5期1110-1113,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81373944)
关键词
穿山龙
多糖
脱蛋白
Dioscorea nipponica Makino
Polysaccharides
Deproteinization