金银花清热解毒作用的血清代谢组学研究

Effect of Heat-Clearing and Detoxifying Health Function of Lonicera japonica in Rats Based on Metabonomics

目的:运用代谢组学方法观察金银花对大鼠血清的影响,研究金银花清热解毒作用的机制。方法:雄性Wistar大鼠连续5 d灌胃给予金银花水提液,通过GC-MS技术分析大鼠血清中小分子代谢组的变化。运用质谱解析、主成分分析等多元线性统计技术分析差异代谢产物,解析内源性代谢产物的变化。结果:大鼠在给药后,α-酮戊二酸、苹果酸及琥珀酸等三羧酸中间产物下降,亮氨酸及异亮氨酸等氨基酸及3-羟基丁酸等脂肪酸代谢的中间产物上升,推测大鼠体内三羧酸循环代谢通路受到抑制,与金银花清热解毒的功效相吻合。结论:代谢组学作为全面评价内源性物质变化的一种方法,可用于金银花的清热解毒机理的评价,为研究中药的功效提供了一种手段。

Objective： To study the metabonomics for heat-clearing and detoxifying health function of Lonicera japonica in rats.Methods： Male Wistar rats were treated with decoction of Lonicera japonica through intragastric for 5 d. GC-MS was used to detect the changes in rats serum metabolites. Mass spectrometry analysis,PCA and other technologies were used to analyze the differences among their metabolites,and analyzed the endogenous product. Results： There was a decline trend in citric acid cycle intermediates of Lonicera japonica treated group. On the contrary,amino acids and fatty acid intermediate product showed significant elevation,which revealed that the pathway of tricarboxylic acid cycle has been inhibited,and consistented with heat-clearing and detoxifying effect of Lonicera japonica. Conclusion： The metabonomics method by detecting the low-molecular-weight compounds can evaluate the heat-clearing and detoxifying health function of Lonicera japonica.