摘要
The utility of microorganisms for solubilizing the unavailable forms of potassium(K) from soil has led to renewed interest in fabrication of rapid and sensitive plate assays for their isolation and screening. The present study developed a modified plate assay and compared it with previously reported methods for the isolation and screening of K-solubilizing bacteria. The newly developed plate assay is based on improved visualization of halo zone formation around the colonies on agar plates, through inclusion of an acid-base indicator dye, bromothymol blue(BTB), to modify the previously reported Aleksandrov medium. The halo zone exhibited a significant correlation(R = 0.939) with K released in liquid medium. The visualization of potential K solubilizers was improved using this method, which would help in detection of weak/non-acid producers based on secretion of organic acids in the medium. Organic acids in plate diffuse radially and form halo zones in response to reaction with the acid-base indicator dye BTB. Furthermore, K solubilization on plates with this method can be observed within 48–72 h, against the incubation time of 4–5 d needed in the earlier method. Therefore, the newly developed protocol for the plate assay was time saving, more sensitive, and beneficial in comparison to the previously reported Aleksandrov plate assay.
The utility of microorganisms for solubilizing the unavailable forms of potassium （K） from soil has led to renewed interest in fabrication of rapid and sensitive plate assays for their isolation and screening. The present study developed a modified plate assay and compared it with previously reported methods for the isolation and screening of K-solubilizing bacteria. The newly developed plate assay is based on improved visualization of halo zone formation around the colonies on agar plates, through inclusion of an acid-base indicator dye, bromothymol blue （BTB）, to modify the previously reported Aleksandrov medium. The halo zone exhibited a significant correlation （R = 0.939） with K released in liquid medium. The visualization of potential K solubilizers was improved using this method, which would help in detection of weak/non-acid producers based on secretion of organic acids in the medium. Organic acids in plate diffuse radially and form halo zones in response to reaction with the acid-base indicator dye BTB. Furthermore, K solubilization on plates with this method can be observed within 48-72 h, against the incubation time of 4-5 d needed in the earlier method. Therefore, the newly developed protocol for the plate assay was time saving, more sensitive, and beneficial in comparison to the previously reported Aleksandrov plate assay.
基金
the Department of Biotechnology (DBT), India
ICAR-Indian Agricultural Research Institute (IARI) for providing financial support
关键词
快速筛选
平板法
细菌
磷钾
酸碱指示剂
测定
改良
研究开发
bromothymol blue （BTB）, Aleksandrov medium, halo zone formation, indicator dye, K solubilizers, microorganisms
