应用变色法筛选斑玉蕈优良杂交子

Application of allochroic method on screening excellent hybridized strains of Hypsizygus marmoreus

【目的】将变色检测方法应用于斑玉蕈优良杂交菌株的选育,缩短育种过程。【方法】采用分光光度计检测变色培养基的颜色变化,计算脱色率(D值)。【结果】变色检测培养基的颜色变化和脱色率(D值)是一致的,即培养基显示黄色时D值是正值,培养基显示蓝色时D值是负值。D值与斑玉蕈主要农艺性状具有紧密联系,D值是正值的菌株平均吃料速度比D值是负值的菌株快,单瓶产量和正常子实体数与D值呈正相关性,畸形菇数与D值呈负相关性。【结论】应用变色检测法可以快速简单地筛选出优良菌株,缩短斑玉蕈育种进程。

[Objective] To shorten the process of screening excellent hybridized strains in Hypsizygus marmoreus. [Methods] We used the allochroic method in this study. [Results] The results showed that the color change of the allochroic medium was concordant with decolorization ratio（D value）, of which the medium was yellow and the D value was positive value, and the medium was blue and the D value was negative value. Furthermore, we found that the D value was related to the main agronomic characters closely, and the mycelial growth of the strains with positive D value was faster than the strains with negative D value, the output per bottle and the number of normal fruit bodies had positive relationship with D value, and the number of abnormal fruit bodies had negative relationship with D value. [Conclusion] Application the allochroic method could screen the excellent hybridized strains quickly and easily, and could shorten the breeding process of H. marmoreus.