Na-K-ATP酶在重症急性胰腺炎大鼠双侧肺组织中的表达及意义

Expression and Significance of Na-K-ATPase at Two Sides of Lung Tissues in Rats with Severe Acute Pancreatitis

目的探讨大鼠肺组织中Na-K-ATP酶mRNA表达水平随重症急性胰腺炎(SAP)进展的变化规律。方法将24只SD大鼠随机分为空白对照组6只和SAP组18只。SAP组大鼠建立SAP模型后,再随机分为SAP-4 h组、SAP-24 h组和SAP-48 h组,每组大鼠数量均为6只。空白对照组于处理4 h时,SAP-4 h组于建模4 h时,SAP-24 h组于建模24 h时,SAP-48 h组于建模48 h时处死大鼠。重复该操作3次以分别检测干湿比、肺泡水清除率(AFC)及左右肺组织中α1、β1 Na-K-ATP酶mRNA的表达水平。结果 1干湿比。与空白对照组比较,SAP-4 h组、SAP-24 h组及SAP-48 h组的肺干湿比均较低(P<0.01);与SAP-4 h组比较,SAP-24 h组的肺干湿比较低(P<0.01),而SAP-48 h组的肺干湿比较高(P<0.01);与SAP-24 h组比较,SAP-48 h组的肺干湿比较高(P<0.01)。2 AFC值。与空白对照组比较,SAP-4 h组及SAP-24 h组的AFC值均较高(P<0.01),但SAP-48 h组的差异无统计学意义(P>0.05);与SAP-4 h组比较,SAP-24 h组和SAP-48 h组的AFC值均较低(P<0.01);与SAP-24 h组比较,SAP-48 h组的AFC值较低(P<0.01)。3α1 Na-K-ATP酶m RNA:与空白对照组对应侧肺组织比较,SAP-4 h组、SAP-24 h组及SAP-48 h组左、右肺组织中,α1 Na-K-ATP酶m RNA的表达水平均较高(P<0.01);与SAP-4 h组对应侧肺组织比较,SAP-24 h组的左肺组织中α1 Na-K-ATP酶m RNA的表达水平较低(P<0.05),右肺组织较高(P<0.01),而SAP-48 h组左、右肺组织中α1 Na-K-ATP酶m RNA的表达水平均较低(P<0.01);与SAP-24 h组对应侧肺组织比较,SAP-48 h组左、右肺组织中α1 Na-K-ATP酶mRNA的表达水平均较低(P<0.01)。空白对照组、SAP-4 h组及SAP-48 h组大鼠左、右肺组织中α1 Na-K-ATP酶m RNA的表达水平比较差异均无统计学意义(P>0.05),而SAP-24 h组大鼠右肺组织中α1 Na-K-ATP酶m RNA的表达水平高于左肺组织(P<0.01)。4β1 NaK-ATP酶mRNA:与空白对照组对应侧肺组织比较,SAP-4 h组、SAP-24 h组及SAP-48 h组左、右肺组织中,β1 Na-K-ATP酶m RNA的表达水平均较高(P<0.01);与SAP-4 h组对应侧肺组织比较,SAP-24 h组左、右肺组织中β1 Na-K-ATP酶mRNA的表达水平均较高(P<0.01),但SAP-48 h组左肺组织与SAP-4 h组比较差异无统计学意义(P>0.05),而右肺组织比较差异有统计学意义(P<0.01),SAP-48 h组较低;与SAP-24 h组对应侧肺组织比较,SAP-48 h组左、右肺组织中β1 Na-K-ATP酶m RNA的表达水平均较低(P<0.01)。空白对照组及SAP-48 h组左、右肺组织中β1 Na-K-ATP酶m RNA的表达水平比较差异均无统计学意义(P>0.05),而SAP-4 h组和SAP-24 h组右肺组织中β1 Na-K-ATP酶m RNA的表达水平高于左肺组织(P<0.05)。结论α1 Na-K-ATP酶mRNA可能是决定SAP大鼠AFC变化的一个重要原因,参与了SAP大鼠肺组织中水的调节。

Objective To discuss the change of expression of Na-K-ATPase mRNA in the rats with severe acute pancreatitis （SAP）. Methods Twenty four SPF SD rats were randomly divided into sham group （n=6） and SAP group （n=18）, the experiment concluded 3 sub-experiments, and each sub-experiments enrolled 24 rats. After establishment of SAP model successfully, rats of SAP group were randomly divided into SAP-4 h group （n=6）, SAP-24 h group （n=6）, and SAP-48 h group （n=6）. Rats of sham group were only conducted the abdominal exploration. Rats of 4 groups were sacrificed （sham group：4 hours after surgery; SAP-4 h group：4 hours after surgery; SAP-24 h group：24 hours after surgery; SAP-48 h group：48 hours after surgery） to determine the dry/wet ratio, alveolar fluid clearance （AFC）, and expressions of Na-K-ATPase mRNA at 2 sides of lung tissues. Results ① Dry/wet ratio. Compared with sham group, the dry/wet ratios of SAP-4 h group, SAP-24 h group, and SAP-48 h group were all lower （P〈0.01）; compared with SAP-4 h group, the dry/wet ratio of SAP-24 h group was lower （P〈0.01）, but dry/wet ratio of SAP-48 h group was higher （P〈0.01）; compared with SAP-24 h group, dry/wet ratio of SAP-48 h group was higher （P〈0.01）. ② AFC. Compared with sham group, the AFC value of SAP-4 h group and SAP-24 h group were both higher （P〈0.01）, but there was no significant difference between sham group and SAP-48 h group （P〉0.05）; compared with SAP-4 h group, the AFC value of SAP-24 h group and SAP-48 h group were both lower （P〈0.01）; compared with SAP-24 h group, the AFC value of SAP-48 h group was lower （P〈0.01）. ③ α1 Na-K-ATPase mRNA. Compared with corresponding side of lung tissue in sham group, the expression level of α1 Na-K-ATPase mRNA at both 2 sides of lung tissues in SAP-4 h group, SAP-24 h group, and SAP-48 h group were higher （P〈0.01）; compared with corresponding side of lung tissue in SAP-4 h group, the expression level of α1 Na-K-ATPase mRNA was lower at left lung tissue （P〈0.05） and was higher at right lung tissue （P〈0.01） in SAP-24 h group, and expression level of α1 Na-K-ATPase mRNA at both sides of lung tissues was lower in SAP-48 h group （P〈0.01）; compared with corresponding side of lung tissue in SAP-24 h group, the expression level of α1 Na-K-ATPase mRNA at both 2 sides of lung tissues was lower in SAP-48 h group （P〈0.01）. There was no significant difference in the expression level of α1 Na-K-ATPase mRNA between left lung tissue and right lung tissue in sham group, SAP-4 h group, and SAP-48 h group （P〉0.05）, but the expression level of α1 Na-K-ATPase mRNA at right lung tissue was higher than that of left lung tissue in SAP-24 h group （P〈0.01）. ④ β1 Na-K-ATPase. Compared with corresponding side of lung tissue in sham group, the expression level of β1 Na-K-ATPase mRNA at both 2 sides of lung tissues in SAP-4 h group, SAP-24 h group, and SAP-48 h group was higher （P〈0.01）; compared with corresponding side of lung tissue in SAP-4 h group, the expres-sion level of β1 Na-K-ATPase mRNA at both 2 sides of lung tissues in SAP-24 h group was higher （P〈0.01）, and lower in right lung tissue of SAP-48 h group （P〈0.01）; compared with corresponding side of lung tissue in SAP-24 h group, the expression level of β1 Na-K-ATPase mRNA at both 2 sides of lung tissues was lower in SAP-48 h group （P〈0.01）. There was no significant difference in the expression level of β1 Na-K-ATPase mRNA between left lung tissue and right lung tissue in sham group and SAP-48 h group （P〉0.05）, but the expression level of β1 Na-K-ATPase mRNA at right lung tissue was higher than that of left lung tissue in SAP-4 h and SAP-24 h group （P〈0.05）. Conclusion α1 Na-K-ATPase mRNA may be the key factor for AFC, and may involved in the water transformation in lung tissue of SD rat with SAP.