重组痘苗病毒TTVAC7理化特性

The physical and chemical characteristics of the recombinant vaccinia virus TTVAC7

探讨重组痘苗病毒TTVAC7的理化特性,对重组痘苗病毒活载体疫苗的规模化生产、保存、运输和净化具有一定的指导意义。将重组痘苗病毒TTVAC7和天坛株痘苗病毒VTT经热、酸、碱、乙醚、氯仿、胰蛋白酶、紫外线照射及超声波破碎处理后,接种于BHK-21细胞,通过测定TCID50观察上述理化因子处理前后TTVAC7和VTT毒价的变化,并分析这些理化因子对TTVAC7和VTT的影响。结果显示,TTVAC7经55℃作用15min,VTT经55℃作用30min即可完全被灭活;在pH值3~9的范围内,pH值的变化不会引起TTVAC7和VTT病毒滴度的显著变化;紫外线垂直距离10、30cm照射病毒液60min,不能完全灭活病毒粒子;对氯仿敏感;经1%胰蛋白酶37℃处理60min,TTVAC7和VTT的病毒滴度下降2个滴度以上;对乙醚不敏感;经40、80Hz的非接触式超声处理60min,TTVAC7和VTT的病毒滴度下降低于2个滴度。结果表明,重组痘苗病毒TTVAC7和天坛株痘苗病毒VTT理化性质相似,均不耐高温,耐酸、碱、乙醚、紫外线及超声波,对氯仿和胰蛋白酶敏感。

To investigate the physical and chemical characteristics of TTVAC7, which has definite guilding sense to the large-scale production, transportation and purification of the recombinant vac- cinia virus live vector vaccine. The BHK-21 cells were infected by the TTVAC7 and VTT,which were treated with heat, acids, alkalis, ether, chloroform, trypsin, ultraviolet rays and ultrasonic waves,and then detected the viral titer of TTVAC7 and VTT before and after treated to analyze the impact of these physical and chemical factors on them. The results showed that TTVAC7 and VTT were inactivated under 55~C for 15 min and 30 min,respectively;The viral titer of TTVAC7 and VTT was not significantly affected in a range of pH 3-9 ~TTVAC7 and VTT exposed to ultra- violet rays at a vertical distance of 10 cm and 30 cm for 60 min were not completely inactivated; TTVAC7 and VTT were sensitive to 5% chloroform. Subjection to 1% trypsin at 37℃ for 60 min of TTVAC7 and VTT reduced the viral titer above 2 logs;TTVAC7 and VTT were not sensitive to 20 % ether;TTVAC7 and VTT exposed to non-contact ultrasonic at 40 Hz and 80 Hz for 60 min leaded to reduce the viral titer below 2 logs. The results indicated that the characterization of TT- VAC7 and VTT were sensitive to heat tochloroform and trypsin, and tolerant of acids,alkalis,ether, ultraviolet rays and ultrasonic waves.