芍药汤高效液相色谱指纹图谱的建立

Establishment of HPLC Fingerprint of Shaoyao Tang

目的:建立芍药汤水煎液的RP-HPLC指纹图谱检测方法。方法:使用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),柱温25℃,流速1.0 m L·min-1,波长230 nm,流动相乙腈-0.05%磷酸水溶液,以芍药苷为参照峰,评价10批芍药汤HPLC指纹图谱。结果:确定了其中13个共有峰,并对其中的3个峰进行指认,分别为芍药苷、黄芩苷、小檗碱,建立了芍药汤水煎液指纹图谱;10批芍药汤HPLC指纹图谱相似度均>0.96。结论:该法灵敏度高、稳定性和准确性良好,体现了芍药汤组分的整体特征,为芍药汤制剂质量标准的建立提供了可靠的科学依据。

Objective： To establish the chromatographic fingerprint for Shaoyao Tang by RP-HPLC.Method： The fingerprint was determined by Agilent ZORBAX SB-C18column（ 4. 6 mm × 250 mm,5 μm）,with the column temperature 25 ℃,the flow rate 1. 0 m L·min- 1,the detective wavelength 230 nm and gradient elution with acetoniteile-0. 05% phosphoric acid as mobile phase. Using paeoniflorin peak as reference, the HPLC fingerprints of 10 batches of Shaoyao Tang were analyzed. Result： Thirteen common peaks were marked and attributed to the herbs,and 3 common peaks were determined by the relative time of the standard substance,they are paeoniflorin,baicalin and berberine respectively. The HPLC fingerprint of Shaoyao Tang was established and the similarity of the 10 batches of samples was above 0. 96. Conclusion： The established HPLC-DAD fingerprint is accurate,stable and reproducible,which could reflect the overall characteristics of Shaoyao Tang and improve the specificity for quality standard of the Chinese herbal formula.