海南红树林淡紫拟青霉EPS纯化物体外抗HSV-1实验研究

Anti-HSV-1 activity in vitro of extracellular polysaccharides purification from Paecilomyces lilacinuson isolated from Hainan mangrove

目的:探讨海南红树林淡紫拟青霉胞外多糖(EPS)纯化物体外抗单纯疱疹病毒Ⅰ型(HSV-1)活性,为开发新型抗病毒药物提供基础研究。方法:以Vero细胞为病毒感染靶细胞,以不同浓度EPS纯化物作用于HSV-1感染过程的各个阶段,以细胞病变程度(CPE)测定病毒半数感染量(TCID5 0),MTT法检测该纯化物对Vero细胞的毒性作用及对HSV-1的直接灭活作用、对病毒吸附和生物合成的影响。结果:EPS纯化物对Vero细胞无增殖作用,其半数有毒浓度(CC5 0)为735.49μg/mL,纯化物在400μg/mL以下时,细胞存活率达90%以上;在所用浓度范围内该纯化物可不同程度抑制病毒吸附,抑制率最高达35.0%,与病毒对照组相比差异具有显著性(P<0.01);该纯化物还具有抑制HSV-1生物合成作用,抑制率与药物浓度呈现出量效关系,其半数抑制浓度(IC5 0)为387.26μg/mL,最高抑制率达61.3%;在所用浓度范围内未发现该纯化物对HSV-1有直接灭活作用。结论:红树林来源的淡紫拟青霉EPS纯化物对Vero细胞毒性较小;该纯化物在一定浓度范围内可抑制HSV-1吸附和生物合成,且抑制生物合成作用较强,并表现出一定量效关系。

Objective： To explore the antiviral activity on HSV-1 of the extracellular polysaccharides （EPS） purification from Paecilornyces lilacinus isolated from mangrove in Hainan province. Methods： The toxicity of the EPS purification on Vero cells and its anti-HSV-1 activity were assessed by cytopathic effect （CPE） and MTT assay. The Veto cells survival rates, HSV-1 inhibition rates by the purification and virus titer were calculated. Results： The purification had little cytotoxic effect on Vero with a CC50 value of 735.49 μg/mL. It could inhibit HSV-1 absorption on Vero cells, and there was a significant differ-ence （P〈0.01） compared with control group （virus group）, and the highest inhibition ratio was 35.0% at dose of 400μg/mL; the biosynthesis of HSV-1 could be inhibited by the extract with dose-dependent manner, and the IC50 value to the viruses was 387.26 μg/mL, and the highest inhibition ratio was 61.3 % at dose of 400 μg/mL;but the purification couldn＇t inactivate HSV- 1 directly. conclusions： The EPS purification had certain antiviral effects it could inhibit HSV-1 absorption and biosynthesis with a dose effect relationship.