瘦素对胃腺癌细胞株SGC7901及结肠癌细胞株HT-29细胞增殖、凋亡的影响

Effect of leptin on cell proliferation and apoptosis of gastric adenocarcinoma cell line-SGC7901 and colon cancer cell line-HT-29

目的:探讨瘦素在胃腺癌细胞株SGC7901及结肠癌细胞株HT-29细胞增殖与凋亡进程中的影响,为胃腺癌及结肠癌临床诊治提供参考依据。方法:采用MTT法及流式细胞仪检测外源性瘦素对胃腺癌细胞株SGC79011及结肠癌细胞株HT-29细胞周期的影响。结果:质量浓度为0、5、50、100、200ng/mL的瘦素具有刺激胃腺癌细胞株SGC79011及结肠癌细胞株HT-29生长的作用;5、50、100、200ng/mL的外源性瘦素作用72h对胃腺癌细胞株SGC7901和结肠癌细胞株HT-29的细胞生长具有明显抑制作用,其中细胞株SGC7901与细胞株HT-29在200ng/mL外源性瘦素作用下其抑制细胞的效应最为明显。结论:在一定浓度和作用时间范围内外源性瘦素具有刺激胃腺癌细胞株及结肠癌细胞株生长的作用,而促进肿瘤细胞增殖和/或抑制肿瘤细胞发生凋亡是其可能的作用机制。

Objective： To discuss the effect of leptin on cell proliferation and apoptosis of gastric adenocarcinoma cell line-SGC7901 and colon cancer cell line-HT-29, and then provided reference basis for gastric adenocarcinoma and colon cancer clinical diagnosis and treatment. Methods： MTT and flow cytometry （FCM） were adopted for detecting the effect of exogenous leptin on cell cycle of gastric adenocarcinoma cell line-SGC7901 and colon cancer cell iine-HT-29. Results： Leptin with mass concentration（0, 5ng/mL, 50 ng/mL, 100 ng/mL, 200 ng/mL） had effects of stimulating the growth of gastric adenocarcino- ma cell line-SGC7901 and colon cancer cell line-HT-29; exogenous teptin with mass concentration（5 ng/mL, 50 ng/mL, 100 ng/mL, 200 ng/mL） had obvious inhibiting effects on cell growth of gastric adenocarcinoma cell line-SGC7901 and colon cancer cell line-HT-29 after 72 h; among which, inhibiting effects of cell line-SGC7901 and cell line-HT-29 were the most obvious un- der the effect of exogenous leptin with mass concentration-200 ng/mL. Conclusions： Within a certain concentration and action time, exogenous leptin had stimulating effects on the growth of gastric adenocarcinoma cell line and colon cancer cell line, and then promoted the tumor cell proliferation and/or inhibited the tumor cell apoptosis.