摘要
目的:提取土人参根的多糖并进行含量测定。方法:采用水提醇沉法提取土人参根多糖,以葡萄糖标准液绘制标准曲线,用苯酚—硫酸法,分光光度法在484 nm波长处测定土人参根多糖的含量。结果:回归方程为y=7.4625x-0.0484,相关系数r=0.9999,平均回收率98.9%,RSD=0.31%(n=5)。结论:土人参根多糖含量为3.7%,方法操作简便、重现性好、准确、可靠,可作为检测土人参根多糖含量的优选方法。
Objective: Extraction of Artemisia capillaries polysaccharide, and content determination of polysaccharide from Talinum paniculatum roots. Methods:Water extracting and alcohol precipitation extraction for content polysaccharides from Artemisia capillaries. Using phenol- sulfuric acid method and Spectrophotometric method to make glucose as standard substance. Results: The regression equation for y = 7. 4625x - 0. 0484, the correlation coefficient r = 0. 9999. The average recovery rate was 98.9 %, RSD = 0.31% ( n = 5). Conclusion:Artemisia polysaccharide Content was 3.7 %. The results show that the proposed method was simple, reproducible and accurate, and reliable
出处
《微量元素与健康研究》
CAS
2016年第5期39-41,共3页
Studies of Trace Elements and Health
基金
广西高校右江流域特色民族药研究重点实验室开放课题(右医科字[2014]2号)
国家中医药管理局"十二五"中医药重点学科中药化学建设项目(国中医药人教发[2012]32号)
广西重点学科药物化学建设项目(桂教科研[2013]16号)
广西高校科技创新能力提升工程建设项目(桂教科研〔2014〕14号)
自治区级创新创业民族医药教学团队项目(桂教高教[2015]93号
桂教高教[2016]6号)
关键词
土人参根
多糖
含量测定
苯酚—硫酸法
Talinum paniculatum lroots
polysaccharide
content
phenol - sulfuric acid method