土人参根多糖提取及含量测定

Determination for the content of polysaccharide and extract of the Talinum paniculatum roots

目的:提取土人参根的多糖并进行含量测定。方法:采用水提醇沉法提取土人参根多糖,以葡萄糖标准液绘制标准曲线,用苯酚—硫酸法,分光光度法在484 nm波长处测定土人参根多糖的含量。结果:回归方程为y=7.4625x-0.0484,相关系数r=0.9999,平均回收率98.9%,RSD=0.31%(n=5)。结论:土人参根多糖含量为3.7%,方法操作简便、重现性好、准确、可靠,可作为检测土人参根多糖含量的优选方法。

Objective： Extraction of Artemisia capillaries polysaccharide, and content determination of polysaccharide from Talinum paniculatum roots. Methods：Water extracting and alcohol precipitation extraction for content polysaccharides from Artemisia capillaries. Using phenol- sulfuric acid method and Spectrophotometric method to make glucose as standard substance. Results： The regression equation for y = 7. 4625x - 0. 0484, the correlation coefficient r = 0. 9999. The average recovery rate was 98.9 %, RSD = 0.31% （ n = 5）. Conclusion：Artemisia polysaccharide Content was 3.7 %. The results show that the proposed method was simple, reproducible and accurate, and reliable