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表面接枝法制备睾酮素分子印迹表面等离子体共振传感器 被引量:4

Molecularly Imprinted Film Grafted from Surface Plasmon Resonance Sensor Chip for Determination of Testosterone
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摘要 构建了一种选择性检测睾酮素的分子印迹表面等离子体共振(Surface plasmon resonance,SPR)传感器。采用紫外光引发表面接枝技术,在固定引发转移终止剂的SPR芯片表面制备了以睾酮素为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂的分子印迹膜(Molecularly imprinted film,MIF)。利用SPR在目标共振角处现场监测使MIF聚合成膜过程更易控。偏振调制-红外反射吸收光谱表征证明MIF接枝成功。原子力显微镜结果显示,MIF表面均匀散布着纳米尺寸的孔穴。利用SPR对2.5×10^(-16)~2.5×10^(-6)mol/L睾酮素进行吸附检测,检出限低至2.5×10^(-16)mol/L,对低浓度和高浓度的睾酮素吸附分段进行线性拟合,得到拟合线性方程分别为y=19.69+1.21x(R^2=0.9913)和y=11.5+0.45x(R^2=0.9895);睾酮素类似物雌二醇、雌三醇和黄体酮的吸附实验结果显示,此印迹膜对模板分子有很好的选择性;5次重复洗脱吸附后,MIF仍保持较好的传感性能,说明此传感器具有较高的稳定性和重复利用性;在人工尿液样品中测得的睾酮素回收率为85.2%~92.8%,说明此传感器可以用于实际样品测定。 A molecularly imprinted film ( MIF) was prepared on the surface plasmon resonance ( SPR) sensor chip for the detection of testosterone. The nanometer imprinted film was synthesized by surface grafting approach. First, the gold sensor chip was modified with an iniferter of benzyl diethyldithiocarbamate to create the “grafting from” polymeric surface. Grafting of the MIF onto the SPR chip was subsequently achieved through ultraviolet light-initiated copolymerization of methacrylic acid ( functional monomer ) and ethylene glycol dimethacrylate ( crosslinker ) in the presence of testosterone as a model template. The template molecules were then removed to form a MIF with specific recognition sites for testosterone. With this iniferter technique , self-aggregation in the reaction solution phase was avoided and grafting polymerization was confined to the exterior of the chip surface. The grafting process was implemented by in situ monitoring with SPR, which permitted the thickness of the film to be easily and strictly controlled. The chip surface modified with a testosterone-imprinted film was characterized by the methods of polarization modulation infrared reflection absorption spectroscopy ( PM-IRRAS ) and atomic force microscopy ( AFM ) . According to the results, testosterone-imprinted film modification of the SPR chip was achieved by the distribution of numerous homogeneous, nanoscale holes on the surface. The testosterone-imprinted film was applied on a SPR sensor chip for the detection of testosterone in the range of 2 . 5 × 10^-16 to 2 . 5 × 10^-6 mol/L in acetonitrile with the lowest determining concentration of 2. 5 × 10^-16 mol/L. Adsorption experiments showed that there were two kinds of binding sites in the MIF, and the linear correlations between the changes in reflectivity and the concentration of testosterone were y=19. 69 + 1. 21x(R2=0. 9913) and y=11. 5 + 0. 45x(R2=0. 9895), respectively. Control experiments utilizing estradiol, estriol, and progesterone as analogues showed impressive selectivity and specificity for testosterone determination. The testosterone-imprinted film reproducibility was evaluated with five cycles of rinsing-rebinding and the RSD was 16 . 8% and 11 . 2% for the SPR angle changes in rinsing and rebinding respectively, demonstrating that the MIF-modified SPR sensor had good reproducibility and repeatability. Finally, the SPR sensor was successfully used to determine testosterone in artificial urine samples with recoveries from 85 . 2% to 92 . 8%.
出处 《分析化学》 SCIE EI CAS CSCD 北大核心 2016年第8期1157-1164,共8页 Chinese Journal of Analytical Chemistry
基金 国家自然科学基金(No.20771015) 高等学校学科创新引智计划(No.B07012)资助~~
关键词 表面等离子体共振 分子印迹膜 睾酮素 表面接枝 Surface plasmon resonance Molecularly imprinted film Testosterone Surface grafting
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  • 1Aguilera R, Becchi M, Casabianca H, Hatton C K, Catlin D H, Starcevic B Pope H G. J. Mass. Spectrom. , 1996, 31(2) : 169-176.
  • 2Choi M H, Kim J N Chung B C. Clin. Chem. , 2003, 49(2) : 322-325.
  • 3Daeseleire E, Vandeputte R, van Peteghem C. Analyst, 1998, 123(12): 2595-2598.
  • 4Moal V, Mathieu E, Reynier P, Malthiiry Y Gallois Y. Clin. Chim. Acta, 2007, 386(1) : 12-19.
  • 5Bricout V, Wright F Lagoguey M. Int. J. Sports Med. , 2003, 24(3) : 197-202.
  • 6Price C P. Clin. Chem. , 1998, 44(10) : 2071-2074.
  • 7Homola J, Yee S S Gauglitz G. Sens. Actuators B, 1999, 54(1) : 3-15.
  • 8Park B, Yun S H, Cho C Y, Kim Y C, Shin J C, Jeon H G, Huh Y H, Hwang I, Baik K Y Lee Y I. Light: Sci. Appl. , 2014, 3(12): e222.
  • 9周春燕,陈长宝,李洁,隋红光,高吉刚,周杰.苏丹红Ⅰ印迹表面等离子共振传感器的制备与表征[J].应用化学,2014,31(9):1107-1114. 被引量:5
  • 10姚婷,李腾飞,秦玉昌,王静,赵祯,谷旭,佘永新.分子印迹表面等离子共振传感器在食品安全检测中的最新研究进展[J].分析测试学报,2015,34(2):237-244. 被引量:15

二级参考文献58

  • 1李金,叶磊.阿片类药物的检测[J].中国药物依赖性杂志,2004,13(3):235-237. 被引量:4
  • 2吴惠勤,黄晓兰,黄芳,林晓珊,邓欣,蔡大川.食品中苏丹红1号的GC-MS/SIM快速分析方法研究[J].分析测试学报,2005,24(3):1-5. 被引量:56
  • 3胡小刚,李攻科.分子印迹技术在样品前处理中的应用[J].分析化学,2006,34(7):1035-1041. 被引量:61
  • 4邓文礼,杨大本,方晔,白春礼.硫醇在Au(111)上SA单分子层膜研究[J].中国科学(B辑),1996,26(2):174-180. 被引量:16
  • 5食品中苏丹红染料的检测方法高效液相色谱法.中华人民共和国国家标准.GB/T19681-2005.
  • 6Gan T,Li K,Wu K.Multi-wall Carbon Nanotube-based Electrochemical Sensor for Sensitive Determination of Sudan Ⅰ[J].Sens Actuators B:Chem,2008,132(1):134-139.
  • 7He L,Su Y,Fang B,et al.Determination of Sudan Dye Residues in Eggs by Liquid Chromatography[JP] and Gas Chromatography mass Spectrometry[J].Anal Chim Acta,2007,594(1):139-146.
  • 8Piao C,Chen L.Separation of Sudan Dyes from Chilli Powder by Magnetic Molecularly Imprinted Polymer[J].J Chromatogr A,2012,1268:185-190.
  • 9L pez M I,Ruis nchez I,Callao M P.Figures of Merit of a SERS Method for Sudan I Determination at Traces Levels[J].Spectrochim Acta,2013,111:237-241.
  • 10Long C,Mai Z,Yang X,et al.A New Liquid-liquid Extraction Method for Determination of 6 Azo-dyes in Chilli Products by High-performance Liquid Chromatography[J].Food Chem,2011,126(3):1324-1329.

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