鱼腥草生物碱抑制人大细胞肺癌细胞生长研究

Study on antitumor activity of houttuynia cordata alkaloids on human large cell lung cancer

目的研究鱼腥草生物碱对人大细胞肺癌细胞株H460生长的抑制作用。方法用20倍95%乙醇回流提取,大孔树脂分离纯化鱼腥草生物碱;MTS实验观察鱼腥草生物碱对H460细胞体外增殖的作用,计算IC50;观察鱼腥草生物碱对H460细胞形态及细胞核的影响,流式细胞仪检测鱼腥草生物碱抑制H460细胞周期及诱导H460凋亡的情况。结果鱼腥草生物碱可抑制H460细胞增殖,IC50为130.17μg/m L。鱼腥草生物碱可致H460细胞萎缩、死亡、细胞核碎裂及出现凋亡小体,能使H460细胞周期阻滞于G0/G1期,诱导H460凋亡。结论鱼腥草生物碱能抑制H460细胞的生长,具有诱导其发生凋亡的作用。

Objective It is to observe the growth inhibition effect of Houttuynia cordata alkaloids on human large cell lung cancer cell line H460.Methods Houttuynia cordata alkaloids been reflux extracted by 20 times of 95% ethanol,separated and purificated by macroporous resin.The effects of Houttuynia cordata alkaloids on proliferation of H460 cells in vitro was observed by MTS experiments,and IC50 was calculated.The effects of Houttuynia cordata alkaloids on H460 cell morphology and nuclear were observed under microscope,and the H460 cell cycle and apoptosis induced by Houttuynia cordata cordata alkaloids were detected by flow cytometer.Results The proliferation of H460 cells were inhibited by Houttuynia cordata alkaloids in vitro,IC50 was 130.17μg / m L.The Houttuynia cordata alkaloids could induced H460 cells atrophy,death,occurring apoptotic bodies and nuclear fragmentation,and made cell cycle arrest in G0/ G1 phase,and induced H460 apoptosis.Conclusion Houttuynia cordata alkaloid can inhibit H460 growth and with the role of induce apoptosis.