15肽介导的聚吡咯修饰的纳米金花对小鼠脏器的损伤及其体内代谢分析

Injury of gold nanoflower modified with 15 peptide-mediated polyrrole on mouse organ and in vivo metabolism of the drug

目的分析15肽介导的聚吡咯修饰的纳米金花(gold nanoflower modified with 15 peptide mediated polyrrole,15P-PPy-NPS)对小鼠脏器的损伤及体内代谢情况。方法向SKOV-3肿瘤荷瘤裸鼠尾静脉注射15P-PPy-NPS,采用电感耦合等离子体质谱(inductively coupled plasma-mass spectrometry,ICP-MS)技术分析不同时间点Au元素在裸鼠血液和主要脏器中的沉积及代谢情况。将裸鼠主要脏器制成石蜡切片,利用TUNEL试剂盒及PI碘化丙啶作核染色双标染色,观察裸鼠主要脏器损伤情况。结果 15P-PPy-NPS通过血液进入裸鼠体内,可被肝脏代谢,少量蓄积未造成明显的组织损伤。结论 15P-PPy-NPS对裸鼠肝脏、肾脏、脾脏等主要脏器未造成明显损伤,可经肝脏代谢,用于生物治疗和光热治疗,具有较好的安全性和可靠性。

Objective To analyze the injury of gold nanoflower modified with 15 peptide mediated polyrrole（15P-PPyNPs） on mouse organ as well as in vivo metabolism of the drug. Methods SKOV-3 tumor-bearing nude mice were injected i. v. with 15P-PPy-NPS, of which the accumulation and metabolism of Au element in blood and major organs at various time points were analyzed by inductively coupled plasma-mass spectrometry（ICP-MS）. The main organs of nude mice were prepared into paraffin sections and observed for the injury by immunofluorescence staining. Results The 15P-PPy-NPs may enter the body of nude mice through the blood, then be metabolized by the liver, and cause a small amount of accumulation with no significant tissue damage. Conclusion The 15P-PPy-NPS showed no injury on the main organs such as liver, kidney and spleen of nude mice, which might be metabolized through liver and used for biological and photothermal therapies, and showed high safety and reliability.