成年人黄斑色素吸光度与血清叶黄素、玉米黄素水平相关性研究

Relationship between macular pigment optical density and serum concentration of lutein and zeaxanthin in an adult population

目的观察成年人黄斑色素吸光度（MPOD）与血清叶黄素、玉米黄素浓度的相关性。方法轻度白内障患者20例和健康成年人39名纳入研究。其中，男性15人，女性44人。平均年龄（43．75±18．93）岁。无吸烟史者53人，有吸烟史者6人，均为男性。偏好动物性食物者2人，偏好植物性食物者22人，平衡膳食者35人。采用双色光闪烁吸光度计法测量黄斑中心凹0．25°、0．50°、1．00°、1．75°MPOD；反相高效液相色谱法测定血清叶黄素、玉米黄素浓度。Pearson相关分析法分析MPOD与血清叶黄素、玉米黄素浓度的相关性。血清叶黄素、玉米黄素浓度与性别、吸烟、饮食习惯的关系。结果受试者黄斑中心凹0．25°、0．50°、1．00°、1．75°MPOD分别为0．59、0．48、0．34、0．18；血清叶黄素、玉米黄素平均浓度分别为（0.45±0．16）、（0．11±0．04）μmol／L。男性血清叶黄素（t=1.13）、玉米黄素（t=0．86）浓度均略高于女性，差异无统计学意义（P=0．27、0．40）。吸烟者血清叶黄素（t=-0．15）、玉米黄素（t=-0．11）浓度略高于非吸烟者，差异无统计学意义（P=0．87、0．91）。偏好动物性食物者血清叶黄素、玉米黄素水平略低于偏好植物性食物或平衡膳食者，三者间差异无统计学意义（F叶黄素=3．87、4．05、0．18，P=0．83、0．81、0．99；F玉米黄素=0．99、1．51、0．52，P=0．85、0．68、0．72）。MPOD与血清叶黄素（r=-0．06、-0．02、-0．07、0．03）、玉米黄素（r=0．02、0．12、0．09、0．11）浓度无相关性（P〉0．05）。结论成年人MPOD与血清叶黄素、玉米黄素浓度无相关。

Objective To study the relationship between macular pigment optical density （MPOD） and serum concentration of lutein and zeaxanthin in an adult population. Methods Twenty patients with mild cataract and 39 healthy subjects were enrolled in this study, including 15 males and 44 females. The average age was 43.75 years. Fifty-three subjects were non-smokers and 6 male subjects were smokers. Two subjects preferred meat diet, 22 preferred meat-less diet, and 35 have balanced diet. MPOD was measured using heteroehromatic flicker photometry at 0.25, 0.5, 1.0 and 1.75 degrees, and serum concentration of lutein and zeaxanthin was measured using high-performance liquid chromatography. The relationship between MPOD and serum concentration of lutein and zeaxantbin was analyzed. The differences of serum lutein and zeaxanthin between different gender, smokers and non-smokers and subjects with different dietary pattern were also analyzed. Results MPOD at 0.25, 0.5, 1.0 and 1.75 degrees were 0.59, 0.48, 0.34 and 0.18, and the average concentration of lutein and zeaxanthin were （0.45 ± 0.16） μmol/L and （0.11±0.04） μmol/L respectively. Serum concentration of lutein and zeaxanthin in males were slightly higher than that in females, but it was not statistically significant （t= 1.13, 0. 86; P=0.27, 0.40）. The differences of serum lutien and zeaxanthin between smokers and non-smokers （t=- 0.15, -0.11; P = 0.87, 0.91）, among subjects of 3 dietary patterns groups were not statistically significant （Flutein=3.87, 4. 05, 0.18; P=0.83, 0.81, 0.99. F zeaxanthin=0.99, 1.51, 0.52; P=0.85, 0. 68, 0. 72）. There was no correlation between MPOD and serum concentration of lutein （r= -0. 06, - 0.02,-0.07,0.03;P〉0.05） andzeaxanthin（r=0.02,0.12,0.09,0.11; P〉0.05）.Conclusion MPOD was not statistically significantly correlated with serum concentration of lutein and zeaxanthin in the studied population.