补肾活血方联合微囊化颗粒细胞移植对大鼠血清FSH、LH及E_2的影响

Effects of Bushen Huoxue Prescription and Transplanting Microencasulated Granulosa Cells on Rats' Serum FSH,LH and E_2

目的:探讨补肾活方联合微囊化细胞移植技术对大鼠生殖内分泌的影响。方法:将50只SD雌性大鼠随机分成5组:空白组(A组)、去卵巢组(B组)、微囊化GC细胞移植组(C组)、低剂量补肾中药+微囊化GC细胞移植组(D组)、高剂量补肾中药+微囊化GC细胞移植组(E组)。采用酶联免疫吸附法(ELISA)检测治疗前后血清雌二醇(E_2)、卵泡刺激素(FSH)及黄体生成素(LH)浓度。结果:微囊化GC细胞移植后,C组大鼠血清E_2水平与B组比较显著升高(P<0.05);FSH、LH水平均明显降低(P<0.01);补肾活血方联合微囊化GC细胞移植后,D、E组E_2水平与C组比较显著升高(P<0.05),FSH、LH水平均明显降低(P<0.05)。结论:与单纯移植微囊化GC细胞相比,补肾活血方联合微囊化细胞移植技术能改善去卵巢大鼠激素分泌状态,其作为临床中医药预防和治疗卵巢早衰的一种手段具有良好的应景前景。

Objective： To investigate the effects of Bushen Huoxue Prescription and transplanting microencasulated granulosa cells on rats reproductive and endocrine systems. Methods：TotallySO SD female rats were randomly divided into five groups：the blank group（ A group）, the model group（ B group）, microencapsulated granulosa cells transplanting group （C group）, the Bushen Huoxue Prescription low dose and microencapsulated granulosa cells transplanting group （D group）, theBushen Huoxue Prescription high dose and microencapsulated granulosa cells transplanting group （Egroup）. Enzyme -linked immune sorbent assay（ELISA） was adopted to detect the concentrations of serum estradiol（ E2 ） ,follicle stimulating hormone （FSH）and luteinizing hormone（LH）. Results：Compared with C group, E21evel in D group was signifi- cantlyincreased （ P 〈 O. 05 ） whereas FSH and LH levels decreased significantly （ P 〈 0. 05 ）. Conclusion ： Compared with simple transplantation of microencapsulated granulosa ceils, Bushen I-Iuoxue Prescriptionand transplantation of micr0en- capsulated granulosa cells in ovariectomized rats can better improve hormone secretion condition, providing a good prospect for Chinese medicine of clinical prevention and treatment of premature ovarian failure.