槐耳清膏对人肺腺癌A549/DDP细胞增殖的影响及顺铂耐药逆转作用

Effect of Huaier on Proliferation and Platinum Resistance of Human Lung Denocarcinoma A549/DDP Cell Line

目的:探讨槐耳清膏对人肺腺癌A549细胞和A549/DDP细胞增殖的抑制影响及其顺铂耐药细胞A549/DDP的逆转作用。方法:以体外培养的人肺腺癌A549及A549/DDP细胞为实验模型,采用MTT法测定不同浓度槐耳清膏对A549细胞及A549/DDP细胞的增殖抑制率,并运用流式细胞术检测顺铂联合槐耳清膏对A549/DDP细胞的变化,以及增敏作用效应。结果:槐耳清膏对A549细胞及A549/DDP细胞的增殖有明显抑制作用,随着药物浓度的增加,其抑制率逐渐增加,具有明显的量-效关系(P<0.05)。顺铂40μmol/L作用于A549及A549/DDP细胞48 h后,其凋亡率分别为(27.61±3.45)%和(17.93±1.73)%,两组间有统计学差异(P<0.01)。槐耳清膏1 mg/m L作用于人肺腺癌A549和A549/DDP细胞40 h后,再以相同浓度顺铂诱导,A549细胞凋亡率为(32.35±2.68)%,A549/DDP细胞凋亡率为(30.01±3.65)%,两组间无统计学意义,但与单独DDP作用相比,差异具有显著意义(P<0.01)。结论:槐耳清膏能显著抑制人肺腺癌A549及A549/DDP细胞的增殖,诱导细胞凋亡,还可以增加A549/DDP细胞对顺铂的敏感性。

Objective ： To observe the effect of Huaier on proliferation and the reversal effect of Huaier on cisplatin - resistant human lung adenocarcinoma cell line Asg9/DDP. Methods ： A model of cell line A549 and A549/DDP of cispla- tin resistance in vitro was built. MTT was used to detect proliferation and flow cytometry （FCM） used to analyze the in- teraction of cisplatin and to A549/DDP cell and sensitization to cisplatin resistance. Results ： Huaier inhibited the prolif- eration of A549 and A549/DDP cells and the inhibition rate increased with the increase of the concentration of Huaier. It was time - and dose - dependent. The inhibition rate of A549 and A549/DDP after 48 hours was different from that in the group of 40 Ixmol/L cisplatin[ （27.61 ±3.45）% vs（17.93 ± 1.73）% ] （P〈0.05）. After 1 mg/mL Huaier inter- vening for 40 hours later, the apoptosis rate of A549/DDP induced by cisplatin and Huaier was （ 30.01 ± 3.65 ） %, not significantly different from that of A549 （32.35 ± 2.68 ） % （ P 〉 0.05 ）, but was significantly different from the group of cisplatin only（ P 〈 0.01 ）. Conclusion： Huaier shows cell proliferation inhibitory and apoptosis inducing effects on human lung adenocarcinoma A549 and A549/DDP cells. Huaier improves the sensibility of A549/DDP cells to cisplatin.