Vav1在胃癌中的表达及其与预后的相关性

The expression of Vav1 and its correlation with prognosis in patients with gastric cancer

目的研究胃癌中Vav1 的表达情况,分析Vav1 表达与胃癌临床病理因素及预后的关系.方法实时定量PCR 检测并比较胃癌细胞株（HGC-27、SGC7901、MGC803）及正常胃黏膜细胞株（GES-1）中Vav1 mRNA 的表达,免疫组化检测105 例胃癌病理组织石蜡标本中Vav1 蛋白的表达情况,分析胃癌患者临床病理学特征与Vav1 蛋白阳性表达间的关系,Kaplan-Meier 法及Cox 回归分析影响胃癌预后的相关因素.结果Vav1 mRNA 的表达在胃癌细胞株（HGC-27、SGC7901、MGC803）明显高于正常胃黏膜细胞株（GES-1）,差异有统计学意义.在胃癌组织标本中,Vav1 蛋白阳性表达与肿瘤直径和淋巴结转移有关（P 〈 0.05）.预后生存分析中,单因素分析显示肿瘤直径、分化程度、浸润深度、淋巴结转移和Vav1 表达与胃癌预后有关（P 〈 0.05）.Cox 回归显示肿瘤浸润深度深（HR=2.764,95%CI 1.316～5.817,P=0.007）,淋巴结转移分级高（HR=1.298,95%CI 1.098～1.534,P=0.002）和Vav1 阳性表达（HR=2.577,95%CI 1.066～3.946,P=0.006）是影响本组胃癌患者预后的危险因素.结论Vav1 在胃癌的侵袭和迁移中发挥着重要的作用,且与胃癌预后密切相关.

Objective To investigate the expression of Vav1 in gastric cancer（GC）, and analyze its potential relevanceto clinicopathological characteristics and prognostic significance in GC patients. Methods The mRNA expression level ofVav1 was detected by real-time quantitative PCR in GC cell lines （HGC-27,SGC7901 and MGC803） and normal gastricmucosa cell line （GES-1）. In addition, the protein expression of Vav1 was analyzed by immunohistochemistry in formalinfixedsamples from 105 GC patients. The associations between clinical pathological features and Vav1 protein expressionwere evaluated in GC patients. Kaplan-Meier method and Cox regression analysis were used to analyze the related factorsinfluencing the prognosis of GC. Results The mRNA expression levels of Vav1 were significantly higher in GC cell lines（HGC-27, SGC7901, MGC803） than those in normal gastric mucosa cell line （GES-1, P〈0.05）. The positive expression ofVav1 in GC tissues was correlated with diameter of tumor and lymph node metastasis （P〈0.05）. The univariate analysisshowed that size of tumor, degree of differentiation, serosal invasion, lymph node metastasis and Vav1 expression weresignificantly related with prognosis of GC （P〈0.05）. Results of Cox regression showed that tumor invasion depth （HR=2.764,95%CI 1.316-5.817, P=0.007）, lymph node metastasis （HR=1.298, 95%CI 1.098-1.534, P=0.002） and Vav1 expression（HR=2.577, 95% CI 1.066- 3.946, P=0.006） were the risk factors affecting prognosis of patients with gastric cancer.Conclusion .