哈萨克马和焉耆马不同组织中IGFBP-5基因表达差异研究

Study on Expression Difference of IGFBP-5 Gene in Different Tissues of Kazakh and Yanqi Horses

试验旨在探求胰岛素样生长因子结合蛋白-5(insulin-like growth factor binding protein-5,IGFBP-5)基因在哈萨克马和焉耆马不同组织部位中的mRNA表达规律。采用实时荧光定量PCR技术检测在哈萨克马和焉耆马心脏、肝脏、脾脏、肺脏、肾脏、小肠、大肠、盲肠、肋间肌、背最长肌、臂肌和臀肌等不同组织中IGFBP-5基因mRNA表达量,同时比较该基因在哈萨克马和焉耆马相同组织中的表达差异。结果表明,哈萨克马和焉耆马表达量最高的均为背最长肌,其次是臂肌,并显著高于心脏、肝脏、脾脏、肺脏、肾脏、小肠、大肠、盲肠等内脏组织表达量(P<0.05),大肠中的表达量最低;焉耆马的肾脏、小肠、大肠、盲肠、背最长肌和臂肌中表达量高于哈萨克马相同部位的表达量,其中焉耆马背最长肌和大肠中的表达量极显著高于哈萨克马的相同部位(P<0.01),小肠和盲肠中的表达量显著高于哈萨克马的相同部位(P<0.05)。本试验为深入研究IGFBP-5基因的生物学功能,以及对中国马生产性能的遗传改良提供理论依据。

The study aimed to explore the mRNA expression pattern of insulin-like growth factor binding protein-5（IGFBP-5）gene in different tissues of Kazakh and Yanqi horses.The expression of IGFBP-5gene in different tissues of heart,liver,spleen,lung,kidney,small intestine,large intestine,cecum,intercostal muscles,longissimus dorsi muscles,brachialis muscle and gluteus in two horses were detected by Real-time quantitative PCR and compared the mRNA expression in the same tissues of two breeds.The results showed that the expression of IGFBP-5in longissimus dorsi muscle and brachialis muscle of two breeds were significantly higher than other tissues including heart,liver,spleen,lung,kidney,small intestine,large intestine and cecum（P〈0.05）,and was the lowest in large intestine.The expression of IGFBP-5in kidney,small intestine,large intestine,cecum,longissimus dorsi muscle and brachialis muscles of Yanqi horse were higher than in the same part of Kazakh horse,and among those in longissimus dorsi muscle and large intestine ofYanqi horse were extremely significantly higher than in the same part of Kazakh horse（P〈0.01）,and in small intestine and cecum of Yanqi horse were significantly higher than in the same part of Kazakh horse（P〈0.05）.The test was for further researching the biological function of IGFBP-5gene,and it could provide a theoretical basis for genetic improvement of production performance of horse in our country.