基于线粒体COI基因对云南切梢小蠹地理种群遗传结构研究

On the population phylogeny of T.yunnanensis based on mitochondrial COI gene

采用线粒体DNA细胞色素c氧化酶亚单位I(COI基因)部分序列,对我国西南地区15个地点共计240个云南切梢小蠹个体进行扩增和测序.研究发现,云南切梢小蠹不同地理种群间在线粒体基因组水平上具有不同程度的多样性,位于云南省中部地区的采样点遗传多样性较为丰富,而西北的丽江和宁蒗遗传多样性比较单一,四川的3个采样地在地理上很近,多样性也较为丰富;研究证实,云南切梢小蠹为单起源,源于云南中部,随后向四周扩散分布;云南切梢小蠹的分布与云南松分布息息相关,二者相辅相成,没有云南松的地方亦没有云南切梢小蠹.

This research uses the partial sequence of the mitochondrial DNA cytochrome c oxidase sub - unit I （COI gene） , PCR amplification and sequencing for 15 locations in southwest China, a total of 240 samples. The a-nalysis reveals that there are different mitochondrial genome levels between different geographic populations. The genetic diversity in the central region of Yunnan province is relatively abundant, but the northwest ones （ Lijiang and Ninglang） are relatively unitary. Three samplings from Sichuan are geographically close, and the diversity is also relatively rich. It also explores their origin and concludes that they come from a single origin in central Yunnan ,then spreading to the surrounding regions. The distribution of Tomicus yunnanensis and that of Pinus yunnanensis are closely related to each other; in other words, they are interdependent.