荧光素酶报告基因法测定飞灰中二噁英类物质

Determination of dioxins in fly ash by chemical activated luciferase gene expression

荧光素酶报告基因法(CALUX)相比高分辨气相色谱/质谱法(HRGC/HRMS)测定二噁英浓度时,具有分析周期短、检测成本低、灵敏度高(皮克水平)、能反映样品的毒性总量且可进行高通量检测等优点。实际应用时,可先用CALUX对样品作初步筛选检测,再将筛选出的阳性样品用化学分析法作进一步定性定量检测。通过对42个垃圾焚烧飞灰样品同时进行HRGC/HRMS和CALUX分析,得到两者的换算系数,从而可以利用快速检测的结果和换算系数来推算HRGC/HRMS的结果。实验表明,CALUX结果与HRGC/HRMS结果具有很好的线性相关性(R=0.96),换算系数为0.332。该换算系数与日本得到的结果不同,表明中国飞灰性质有别于日本飞灰。而且,不同环境介质之间换算系数也不一致。为增加该方法在中国的适用性,必须通过实验方法找出符合中国样品的换算系数。

Chemical activated luciferase gene expression （CALUX） holds the following advantages over high resolution gas chromatography/high resolution mass spectrometry （HRGC/HRMS） in PCDD/Fs analysis： （1） faster analysis turnaround, （2） less expensive, （3） high sensitivity with picogram level, （4） detecting the total toxicity, （5） high throughput screening. In practical application, the CALUX bioassay methods can be firstly applied to detect all samples. The positive samples are screened and will be reanalyzed with current chemical analytical technique to conduct the qualitative and quantitative determination. In this study, 42 fly ash samples were simultaneously determined by HRGC/HRMS and CALUX bioassay. The HRGC/HRMS results could then be extrapolated by CALUX results using the conversion coefficient. The results showed that CALUX and HRGC/HRMS were significantly correlated （R = 0.96）. The conversion coefficient between these two analytical techniques was calculated to be 0.332. Different conversion coefficients between China and Japan indicated the special properties in Chinese fly ash. In addition, the conversion coefficients also differed with various matrixes. Considering the applicability of this method in China,a se-ries of experiments should be carried out to identify the conversion coefficients in various matrixes.