载药纳米磷酸钙的制备及其抗菌性能研究

Preparation of arag-corried nano-calcium phosphate and its antibacterial properties

纳米磷酸钙(nCaP)广泛用作骨修复材料,但当其植入体内时,常常发生细菌感染,从而诱发一系列难以治疗的骨科疾病,如骨髓炎等。基于有机双膦酸(NDBP)作为改性剂制备得到的不同结晶度的磷酸钙(CaP)为基础,通过原位法将抗菌药物环丙沙星(CFX)负载在所制备的nCaP中,研究了nCaP的结晶度和NDBP的用量对于CFX的载药率以及药物释放的影响。研究发现随着nCaP结晶度增大或者NDBP加入量减少,CFX的载药率趋于增大,药物释放速度则是逐渐变慢;通过动力学研究发现未加入NDBP,结晶度较高的nCaP对于CFX的药物释放主要受扩散机理控制;而加入NDBP后,nCaP对于CFX的药物释放受不规则扩散机理(non-Fickian)控制,即多种机理控制,包括扩散、溶蚀和解吸附的药物控制机理。

Calcium phosphate nanoparticles（ nCaP） were widely used as bone repair material. A main obstacle to the applications of nCaP was the biomaterial centred infection（ BCI） ,which further caused a series of orthopedic diseases such as osteomyelitis. In order to render the nCaP with antibacterial properties, ciprofloxacin （ CFX） was loaded into nCaP with different crystallinity by using bisphosphate（ NDBP） as surface modifier. The effects of crystallinity and content of NDBP on drug loading and drug release are in-vestigated. It is demonstrated that the drug-loading capacity increased with the increasing of nCaP crystallinity and the decreasing content of NDBP. In addition, lower CFX release rate was observed at higher nCaP crystallinity and lower content of NDBPs. Finally, the release mechanism was studied and presented.