响应面试验优化微波法提取刺玫籽原花青素的工艺

Optimization of Microwave-Assisted Extraction of Proanthocyanidins from Rosa davurica Pall. Seeds by Response Surface Methodology

利用响应面分析,对微波法提取刺玫籽中原花青素工艺进行优化。采用铁盐催化法测定原花青素质量分数,结果表明刺玫籽中原花青素总含量为(1.55±0.12)g/100 g。单因素试验结果表明,六偏磷酸钠添加量为0.2%时能达到最佳稳定效果。在此基础上,选取提取时间、料液比、微波功率、乙醇体积分数为自变量,以原花青素提取率为响应值,采用Box-Behnken设计方法,研究各因素及其交互作用对原花青素提取率的影响。结果表明,最佳工艺参数为:提取时间70 s、料液比1∶20(g/mL)、微波功率360 W、乙醇体积分数60%。经验证,单次提取原花青素的提取率为72.58%,与预测值73.46%相比,相对误差为1.12%,表明优化工艺参数可靠。提取次数为3次时,原花青素提取率可以达到93.19%。

The microwave-assisted extraction of proanthocyanidins（PC） from Rosa davnrica Pall. seeds was optimized using the response surface methodology. The PC content of Rosa davnrica Pall. seeds was determined to be（1.55 ± 0.12） g/100 g by iron salt catalysis method. The results of one-factor-at-a-time experiments showed that the addition of 0.2% sodium hexametaphosphate（SH） provided the best stability for the proanthocyanidins. Extraction time, solid to liquid ratio, microwave power, and ethanol concentration were selected as independent variables, while the extraction yield of PC was taken as response variable. The optimization was carried out based on Box-Behnken experimental design by investigating the individual and combined effects of the four factors on the response variable. The optimum extraction conditions were determined as follows： extracting time, 70 s; material to liquid ratio, 1：20（g/mL）; microwave power, 360 W; and ethanol concentration, 60%. Under these conditions, the PC yield in one extraction cycle obtained was 72.58% with a relative error of 1.12% as compared with the model prediction of 73.46% indicating that these parameters are reliable. In first three cycles, 93.19% of the measured PC were extracted.