木薯茎杆基质比例对3种食用菌海藻糖含量的影响

Effect of Cassava Stalk Substrate Ratio on Trehalose Content in Three Cultivated Edible Fungi

以木薯茎杆栽培的食用菌为试材,对海藻糖的提取方法和高效液相色谱-蒸发光散射(high performance liquid chromatography-evaporative light scattering detector,HPLC-ELSD)检测方法进行优化,分析不同比例木薯茎秆栽培3种食用菌(黑木耳、平菇、榆黄蘑)海藻糖含量。结果表明,食用菌中的海藻糖在80℃水浴60 min条件下可有效提取,最高含量达到190.5 mg/g;利用优化后色谱条件分析标准品和样品中海藻糖,其分离效果均良好。并分析黑木耳、平菇和榆黄蘑中海藻糖含量,发现当栽培基质的木薯茎秆比例为31.2%时,3种食用菌海藻糖含量较高,分别为61.5、250.3 mg/g和15.0 mg/g。结果表明,HPLC-ELSD可分析3种食用菌中海藻糖含量,方法准确度高、重复性好、样品稳定性较好;3种食用菌海藻糖含量与培养基质中木薯茎秆比例有关,合适的木薯茎秆比例可提高食用菌中海藻糖含量。

In this study, an optimized method using high performance liquid chromatography-evaporative light scattering detector（HPLC-ELSD） was developed for detecting trehalose extracted from three edible fungi including Auricularia auricula-judae, Pleurotus ostreatus, and Pleurotus citrinopileatus Sing., which were cultivated on mixed substrates containing assava（Manihot esculenta） stalk. The relationship between trehalose content and cassava stalk proportion was explored. The results indicated that the trehalose in edible fungi could be completely extracted in 80 ℃ water bath in 60 minutes, and the maximum yield of trehalose could reach 190.5 mg/g. Under the optimized chromatographic conditions, good separation of trehalose and maximum contents of trehalose were obtained from edible fungi cultivated on a substrate with 31.2% cassava stalk, which were 61.5, 250.3 and 15.0 mg/g for Auricularia auricula-judae, Pleurotus ostreatus, and Pleurotus citrinopileatus Sing., respectively. This study has demonstrated that the trehalose content of edible fungi can be accurately and conveniently determined by HPLC-ELSD. In addition, the trehalose contents of A. auricula-judae, P. ostreatus and P. citrinopileatus can be significantly improved by cultivating them on a substrate with a proper ratio of cassava stalk.