特异性免疫治疗哮喘患儿NK细胞活性和嗜碱性细胞表达变化的影响

The effect of specific immunotherapy on NK cell activity and the expression of basophil in children with asthma

目的探讨特异性免疫治疗哮喘患儿自然杀伤细胞（natural killer cell，NK）活性和嗜碱性细胞表达变化的影响。方法回顾性分析本院2012年12月至2014年12月收治的97例哮喘患儿的临床资料，按照治疗方法分为对照组（47例）和观察组（50例），分别给予常规治疗和特异性免疫治疗。观察两组的NK细胞活性和嗜碱性细胞表达变化，并进行比较。结果治疗前，两组患者的NK细胞活性与嗜碱性细胞表达水平经比较差异无统计学意义（P〈0．05）；经过不同的治疗之后，观察组和对照组的NK细胞活性分别为（12．12±1．51）％、（10．10±2．10）％，较之本组治疗前均得到显著的提高（P〈0．05）；治疗后两组比较差异有统计学意义（P〈0．05）。观察组和对照组的嗜碱性细胞表达水平分别为（0．41±0．11）％、（0．51±0．15）％，较之本组治疗前均得到显著的下降（P〈0．05）；治疗后两组比较差异有统计学意义（P〈0．05）。结论特异性免疫治疗哮喘患儿可以显著增强其NK细胞活性，并降低嗜碱性细胞的表达水平。

Objective To investigate the effect of specific immunotherapy on natural killer cell （NK cell） activity and the expression of basophil in asthmatic children. Methods A retrospective analysis was performed on clinical data of 97 children with asthma in our hospital from December 2013 to December 2014, and they were divided into control group （47 cases） and observation group （50 cases） according to treatment methods, and were given conventional therapy and specific immunotherapy respectively. The changes of NK cell activity and the expression of basophil in two groups were observed and compared. Results Before treatment, there were no statistically significant differences in NK cell activity and the expression of basophil between two groups （P〉0.05）; after treatment, NK cell activity in observation group and control group were （12.12＋1.51）% and （10.10~2.10）% respectively, improved significantly compared with before treatment （P〈0.05）, with statistically significant difference between two groups （P〈0.05）; the expression of basophil in observation group and control group were （0.41±0.11）% and （0.51±0.15）% respectively, decreased significantly compared with before treatment （P〈0.05）, with statistically significant difference between two groups （P〈0.05）. Conclusion Specific immunotherapy can significantly enhance NK cell activity in children with asthma, and reduce the expression level of basophil.