COX2促进卵巢癌细胞迁移及相关机制的研究

The effects and related mechanisms cyclooxygenase-2 act in the migration of ovarian cancer cells

目的:探讨COX2对卵巢癌细胞株增殖及迁移的影响及其相关机制。方法:构建慢病毒载体Lenti-COX2-EGFP,转染卵巢癌细胞株SKOV3和ES2。PCR及Western blot法鉴定转染效率,CCK-8实验检测细胞增殖,划痕实验观察细胞迁移能力,qRTPCR法检测E-cadherin、Vimentin、Snail和Slug表达。结果:与对照组相比,过表达COX2后卵巢癌细胞的增殖和迁移能力增强,差异均有统计学意义(P<0.05);E-cadherin表达下调,Snail、Slug、Vimentin表达上调。COX2抑制剂塞来昔布处理则可抑制COX2过表达细胞的增殖及迁移能力,与对照组比较差异有统计学意义(P<0.05)。结论:COX2可促进卵巢癌细胞增殖及上皮间质转化(EMT)继而增强肿瘤细胞迁移能力;塞来昔布可发挥阻断作用。推测COX2可能是卵巢癌临床治疗的一个潜在靶点。

Objective： To examine whether COX2 could accelerate proliferation and metastases of ovarian cancer cells and its related mechanisms. Methods： We created LentiCOX2-EGFP and then transfected ovarian cancer cells. After that,we evaluated the effect of COX2 on proliferation and metastases of ovarian cancer via PCR,Western blot,qRT-PCR,CCK-8 test and healing test. Results： Compare with control group,after up-regulating COX2 expression,cell proliferation and migration accelerated. The expressions of Snail and Slug up-regulated,resulting in E-cadherin expression down-regulating and Vimentin expression up-regulating.Celecoxib can inhibit these affections. All of the differences were significant. Conclusion： COX2 can accelerate cell proliferation and EMT,which can accelerate migration. Celecoxib can inhibit this effect. So,COX2 may serve as a potential target for curing ovarian cancer.