Hsa-miR-6841-3p对宫颈癌细胞系Caski增殖及侵袭转移的影响

Effect of miRNA-6841-3p on proliferation,migration and invasion of human cervical cancer cells

目的:探讨miRNA-6841-3p对宫颈癌细胞生物学行为的影响。方法:将miRNA-6841-3p模拟物、miRNA-6841-3p抑制物转染至宫颈癌细胞系Caski。实时荧光定量PCR检测宫颈癌细胞系Caski转染后miRNA-6841-3p mRNA以及其预测靶基因TFF3的转录表达。CCK-8法检测细胞增殖能力。Transwell小室检测细胞体外侵袭转移能力。划痕实验检测细胞迁移能力。结果:实时荧光定量PCR显示,miRNA-6841-3p模拟物转染组与miRNA-6841-3p模拟物对照组相比,miRNA-6841-3p mRNA表达明显上调(P<0.01),miRNA-6841-3p抑制物转染组miRNA-6841-3p mRNA转录表达明显受抑制,差异有统计学意义(P<0.01)。其预测靶基因TFF3 mRNA转录表达在miRNA-6841-3p模拟物转染组明显低于抑制物转染组(P<0.01)。与miRNA-6841-3p抑制物转染组比较,转染miRNA-6841-3p模拟物后,宫颈癌细胞系Caski细胞活性、迁移侵袭能力明显减弱(P<0.05)。结论:上调miRNA-6841-3p表达可明显抑制宫颈癌细胞系Caski增殖,抑制其迁移、侵袭能力,其作用机制可能通过抑制预测靶基因TFF3表达实现。

Objective： To explore the effect of microRNA-6841-3p（ miRNA-6841-3p）on the biological behavior of human cervical cancer cells. Methods： The cervical cancer cell line Caski was transiently transfected with miRNA-6841-3p mimics and miRNA-6841-3p inhibitor by ribo FECT CP. The expression of trefoil factor 3（ TFF3） and miRNA-6841-3p in human cervical cancer cell line Caski were detected by real-time fluorescent quantitative polymerase chain reaction（ RT-PCR）. Cell proliferation was evaluated using the cell counting Kit-8（ CCK-8） assay. Invasion was measured by transwell chamber assays. The wound healing model was used to represent the migrationability. Results： The Ca Ski cells transfected with miRNA. miRNA-6841-3p mRNA expression in cells transfected with miRNA-6841-3p mimics was significantly higher than that in negative control cells（ P〈 0. 01）. miRNA-6841-3p mRNA expression in cells transfected with miRNA-6841-3p inhibitor was significantly lower than that in negative control cells（ P〈 0. 01）. However,the TFF3 mRNA expression in the mimics transfected cells decreased significantly. The overexpression of miRNA-6841-3p inhibited the viability,and invasion and migration abilities,as shown in the cells transfected with the miRNA-6841-3p mimics（ P 〈0. 05）. Conclusion： Overexpression of miRNA-6841-3p had an inhibitory effect on cell proliferation,and markedly inhibited invasion and migration of Caskicells in vitro and in vivo.