无偿献血者HCV RNA与抗-HCV及ALT检测结果的相关性

Relationship between anti-HCV,HCV RNA and ALT in Volunteer Blood Donors

目的：讨论无偿献血人群丙型肝炎病毒抗体（抗-HCV）、HCV RNA、丙氨酸氨基转移酶（ALT）检测结果之间的相关性。方法采用ELISA法检测抗-HCV阳性标本235份，荧光定量PCR法检测病毒RNA，速率法测定ALT，并对抗-HCV（＋）/HCV RNA（–）标本进行追踪回访。结果235份抗-HCV阳性标本中，HCV RNA阳性140例（阳性率59.57%）；抗-HCV S/CO值1～5实验组和5.01～9.99实验组，HCV RNA阳性率分别为34.29%、26.47%；S/CO≥10时，HCV RNA阳性率为81.68%，与其他2组的差异有统计学意义（χ^2=45.15，P〈0.05；χ^2=39.41，P〈0.05）。抗-HCV （＋）/HCV RNA（＋）与抗-HCV（＋）/HCV RNA（–）组的ALT异常率分别为2.86%、1.05%，两组的差异无统计学意义（χ2=0.88，P＆gt;0.05）。抗-HCV（＋）/HCV RNA（＋）与抗-HCV（＋）/HCV RNA（–）组献血者的年龄分别是38.05±11.35岁和33.81±11.50岁，两组的差异有统计学意义（t=2.79，P〈0.05）。95份抗-HCV（＋）/HCV RNA（–）标本成功回访23例，其中1份标本回访检测抗-HCV转阴。结论 HCV RNA阳性率与抗-HCV的S/CO值有一定相关性，抗-HCV阳性的无偿献血人群中，ALT异常率与HCV RNA无相关性，感染者年龄与HCV RNA有一定相关性。

Objective To investigate the relationship between anti-HCV,HCV RNA and ALT in volunteer blood donors. Methods Fluorescent quantitative PCR was used to detect viral loads and kinetic method was employed to assay ALT levels in 235 anti-HCV positive samples who were tested by ELISA. A six month follow up was performed to monitor the anti-HCV（＋）/HCV RNA（–） samples. Results Among 235 anti-HCV positive samples,140（59.57%） were positive for HCV RNA. The HCV RNA positive rate in groups of S/CO ratio 1-5 and 5.01-9.99 were 34.29%and 26.47%, respectively. The HCV RNA positive rate in group of S/CO ratio≥10 was 81.68% and statistically significant differences were found between this and the other two groups（χ^2 =45.15,P〈0.05;χ2=39.41,P〈0.05）. The ALT abnormality rates of anti-HCV（＋）/HCV RNA（＋） and anti-HCV（＋）/HCV RNA（–） groups were 2.86% and 1.05%, respectively. No statistically significant difference was found between them（χ^2=0.88,P≥0.05） . The age of donors with anti-HCV（＋）/HCV RNA（＋）were older than those with the anti-HCV（＋）/HCV RNA（–） （χ^2 =7.81,P〈0.05）. Among 95 blood donors,23 received retrospective survey and the anti-HCV results of one sample was negative in the second detection. Conclusion The positive rate of HCV RNA has relationship with the S/CO ratio of anti-HCV and the age of HCV infected blood donors. No correlation was found between the unqualified rate of ALT and HCV RNA in anti-HCV positive volunteer blood donors.