几种血红蛋白测定方法的比较

Comparisons of Several Methods for Hemoglobin Determination

目的选用较可靠的定量测试法测定献血者血红蛋白浓度,进一步提高献血者血红蛋白检测的精准率和可靠性。方法以血红蛋白检测的金标准法氰化高铁(Hi CN)法为基准,应用血细胞分析仪为对照组,对血红蛋白目测试剂(硫酸铜比重法)和干式血红蛋白检测试纸进行对比试验。结果选择置信度P=95%对151例数据样本进行回归分析,优利特检测(y)与Sysmex基准(x)比较,y=0.892 2x+15.979,R^2=0.7461;Hemocue检测(y)与Sysmex基准(x)比较,y=0.949 4x+15.018,R^2=0.838 1。结论采用干化学法检测血红蛋白,在灵敏度和稳定性方面均能与血细胞分析仪相媲美,相应的精密度、准确度及线性完全符合检测需求,特别针对血站的初筛检验具有实际意义。

Objective To choose a more reliable method to determine the hemoglobin concentration of blood donors in order to further improve the accuracy. Methods On the basis of gold standard method for the detection of hemoglobin （HiCN method）, we used blood cell analyzer as the control group. Comparison test was carried out between visual reagent on hemoglobin （copper sulfate proportion） and dry hemoglobin test paper. Results Regression analysis of 151 cases of data samples with confidence degree P =95% was carried out. Comparison of Uritest detection（y） and Sysmex reference（x）, y=0.892 2x＋15.979, R2=0.7461; comparison of Hemocue detection（y） and Sysmex reference（x）, y=0.949 4x＋15.018, R2=0.838 1. Conclusion Determination of hemoglobin with dry hemoglobin test paper is a method with similar sensitivity and stability with that of the method using blood cell analyzer. The precision, accuracy and linear degree fully meet the needs of testing. It has practical significance in especially blood screening test in blood banks.