野生小豆和栽培小豆microRNA全基因组鉴定与比较分析

Global Identification and Comparison of MicroRNA in Wild and Cultivated Adzuki Bean

MicroRNA(miRNA)是一种非编码的小RNA分子,对真核生物基因表达起着非常重要的调控作用,miRNA系统鉴定对于研究其功能及作用机制具有重要意义。本研究分别以栽培小豆京农6号(JN6)和野生小豆CWA108为试验材料,进行了深度miRNA测序。系统鉴定和注释这2个物种的miRNA,并分析了其miRNA种类的异同、表达量的差异和靶基因在功能富集上的差别。鉴定出了JN6和CWA108共有和特有的miRNA,明确了它们之间差异表达的miRNA,以及这些特有和差异miRNA的靶基因在功能富集上的差异,发现可能和抗病与抗逆途径相关。

MicroRNAs（miRNAs） are a class of small regulatory RNAs with the function as crucial regulators that repress the expression of their target genes at the transcriptional or post-transcriptional level. Global identification of miRNA is important for the functional study. Adzuki bean（Vigna angularis） genome sequencing has been finished recently, but lack of a systematical analysis of miRNA. In this study, we performed deep miRNA sequencing in wild（CW108） and cultivated（JN6） adzuki beans. Our investigation not only identified the miRNAs that shared by these two species, but also found miRNAs that specifically existed in individual accession. Through comparison of the miRNA expression and their target gene predictions between the wild and cultivated adzuki beans, we found that the miRNA was diverged between them, which may be associated with plant-pathogen interaction pathway. Our study provided a valuable resource for functional analysis in adzuki bean.