Bacillus amyloliquefaciens SY07产α-葡萄糖苷酶抑制剂液态发酵条件优化

Optimization of Liquid Fermentation Conditions for the Production of α-Glucosidase Inhibitors by Bacillus amyloliquefaciens SY07

对一株解淀粉芽孢杆菌(Bacillus amyloliquefaciens SY07)产α-葡萄糖苷酶抑制剂的最适培养基和液态发酵条件进行优化。豆渣可作为该菌产α-葡萄糖苷酶抑制剂的适用培养基,优化发酵条件为豆渣5%,装液量60 mL/250 mL三角瓶,接种量2%,初始pH值6.5,摇床转速180r/min,培养温度37℃,发酵时间48 h。在此条件下,发酵产物对α-葡萄糖苷酶活性的抑制率为60.08%。B.amyloliquefaciens SY07良好地利用了豆渣产α-葡萄糖苷酶抑制剂的能力,为潜在的降血糖功能性食品的研发及农副产品的综合利用开辟了新途径。

The optimal medium and liquid fermentation conditions are optimized for the production of α-glucosidase inhibitors by Bacillus amyloliquefaciens SY07. The results show that bean dregs are used as the medium for the strain to generate α-glucosidase inhibitors. The fermentation conditions are optimized as follows.bean dregs of 5%, liquid volume of 60 mL/250 mL, inoculation amount of 2%, initial pH of 6.5, rotation speed of 180 r/min, temperature of 37 ℃ ,and fermentation time of 48 h.Under these conditions, the α-glucosidase inhibitory activity of fermented sample reaches 60.08%. The strain B. amyloliquefaciens SY07 possesses good capacity of producing α-glucosidase inhibitors using bean dregs as medium, which has provided new ways for the development of potential functional foods with hypoglycemic effect as well as the comprehensive utilization of agricultural and sideline products.