宽体金线蛭油酸的气相色谱指纹图谱

Gas Chromatographic Fingerprint of Oleic Acid of Whitmania pigra Whitman

【目的】建立宽体金线蛭油酸的气相色谱指纹图谱。【方法】以不同来源地和不同规格的共10批宽体金线蛭为研究对象,选择Agilent 6890N型气相色谱仪,DB-WAX色谱柱（30 mm×0.32 mm×0.25μm）,气化温度为270℃,柱温130℃,检测器（FID）温度为280℃。采用中国药典委员会颁布的《中药色谱指纹图谱相似度评价系统2004A》进行相似度计算。【结果】不同规格的宽体金线蛭油酸含量差异有统计学意义（F2,7=7.350,P=0.019）,清水晒干品分别和矾品、饮片/晒干的油酸含量比较差异有统计学意义（P=0.021,P=0.009）。指纹图谱的相似度在0.458~0.998之间,其中与其他样品和对照指纹图谱的相似度比较,来源于广西荔浦的样品相似度为最低。【结论】大部分样品指纹图谱的相似度非常高,所建立的气相色谱指纹图谱可有效鉴别宽体金线蛭的优劣品,该方法可为宽体金线蛭的日常质量控制提供一定参考。

Objective To establish the gas chromatographic（GC） fingerprint of oleic acid of Whitmania pigra Whitman for its quality control. Methods Ten batches of Whitmania pigra from different sources and processed by different methods were analyzed with Agilent 6890 N gas chromatography detector on DB-WAX（30 mm × 0.32 mm × 0.25 μm）column at the vaporizing temperature of 270℃,column temperature of 130℃ and flame ionization detector（FID） temperature of 280℃. We used a software of Similarity Evaluation System for Chromatographic Fingerprint of TCM（Version of 2004A） published by Chinese Pharmacopoeia Commission to calculate GC similarity. Results Oleic acid content of Whitmania pigra processed by different methods had significant differences（F2,7= 7.350, P = 0.019）. The oleic acid content of samples dried after washing with clean water significantly differed from that of the samples processed by alumen or the slices dried naturally（P = 0.021,P= 0.009）. The similarity of the fingerprints was in the range of 0.458- 0.998. The similarity of samples from Lipu of Guangxi Province was the lowest. Conclusion The fingerprints of most of the samples have very high similarity. The established GC fingerprints can be used to effectively identify the qualified or inferior Whitmania pigra products,which will provide some reference for the quality control of Whitmania pigra.