中青年高血压病痰湿壅盛证患者尿液代谢组学分析

Analysis on Metabolomics of Urine Samples in Young and Middle-aged Patients with Hypertension of Phlegm Dampness Retention Syndrome

目的寻找中青年高血压病痰湿壅盛证患者的尿液生物标记群,探索其证候本质。方法运用1H NMR结合PLS-DA的方法对中青年高血压病三种证型患者及健康志愿者的尿液进行代谢组学分析,得出生物标记物群。结果中青年高血压病痰湿壅盛组与对照组、肝火亢盛组、阴虚阳亢组对比,痰湿壅盛组代谢物存在显著性变化,主要与三大代谢及嘌呤代谢关系密切。结论运用核磁共振代谢组学方法分析尿液代谢组学,可对中青年高血压病痰湿壅盛患者和健康者、肝火亢盛组、阴虚阳亢组进行区分。

Objective To analyze the urine samples of young and middle-aged patients with hypertension of phlegm dampness retention syndrome by means of metabonomics,and to confirm its biomarker. Method Through the 1HNMR technique combined with partial least squares-discriminate analysis（ PLS-DA） method,the biomarkers were selected. Result The compounds had significant differences between young and middle-aged patients with hypertension of phlegm dampness retention group and the control group. Conclusion Metabonomics might provide a clear physical basis for distinguishing young and middle-aged patients with hypertension of phlegm dampness retention syndrome and liver-fire hyperactivity syndrome,yin deficiency and yang hyperactivity syndrome,healthy people.