白芍总苷对EAT小鼠Th1/Th2型细胞因子表达的影响

Influences of TGP on expression of IFN-γ and IL-4 of mice with EAT

目的:观察不同浓度白芍总苷(total glucosides of paeonia,TGP)对实验性自身免疫性甲状腺炎(experimental autoimmune thyroiditis,EAT)小鼠Th1型细胞因子(IFN-γ)和Th2型细胞因子(IL-4)表达的影响。方法:在建立小鼠EAT模型第3周起灌胃给药,给药组分别给予不同浓度TGP混悬液和雷公藤多苷片混悬液,模型对照组给予等量的0.5%羧甲基纤维素钠(CMC-Na)。用流式细胞仪分析各组小鼠脾细胞分泌IFN-γ和IL-4的T细胞比例。结果:雷公藤多苷和高剂量TGP可显著抑制EAT小鼠脾细胞IFN-γ和IL-4的过高表达,而中、低剂量TGP抑制作用较差。结论:TGP的治疗作用可能与调节EAT小鼠亢进的细胞免疫和体液免疫功能及平衡细胞因子表达有关。

AIM： To observe the expression of Th1 type cytokines（ IFN-γ） and Th2 type cytokines（ IL-4） on experimental autoimmune thyroiditis（ EAT） mice by the different concentrations of total glucosides of Paeonia（ TGP）. METHODS： After establishment of model in the third week,each medicine group was given different concentrations of TGP suspension or solution of triptergium glycoside. The model group was treated by 0. 5% sodium carboxymethyl cellulose（ CMC-Na） in the same volume.Those T cells secreting IFN-γ or IL-4 were analyzed by flow cytometry. RESULTS： Triptergium glycoside and high-dose TGP can apparently inhibit the excessive expression of IFN-γ and IL-4 in EAT mice splenocyte,while inhibitory effects of middle and low-dose TGP are weaker. CONCLUSION： TGP＇s therapeutic effect on EAT mice is related to the regulation of EAT mice＇s cell immune and humoral immune function and the expression of the cytokines.