硫化氢延迟预处理抑制大鼠心肌损伤的机制研究被引量：1

An Investigation on the Mechanism of Hydrogen Sulfide-Induced Delayed Preconditioning on Myo- cardial Ischemia Reperfusion Injury in Rats

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摘要 [目的]探讨硫化氢预处理对大鼠心肌缺血再灌注损伤的保护机制。[方法]健康成年S-D雄性大鼠30只，随机分成三组，每组10只。假手术组（S组）仅开胸并分离冠状动脉左前降支，但不阻断血流；缺血再灌注组（IR组）行冠状动脉左前降支阻断30min，再灌注120min；硫化氢组（H组）予以静脉注射硫氢化钠0．05mg／kg，给药后24h同IR组处理。再灌注结束后检测血清IL-6和IL-10水平，免疫印迹法检测心肌过氧化物酶体增殖物激活受体γ（PPAR-γ）水平并比较。[结果]与s组比较，IR组和H组在缺血再灌注后血清IL-6和IL-10水平升高（P〈0．05）；但与IR组比较，H组IL-10水平升高，IL-6水平下降（P〈0．05）；与S组比较，IR组和H组PPAR-γ表达下降（P〈0．05）；但与IR组比较，H组PPAR7表达升高（P〈0．05）。[结论]硫化氢预处理抑制大鼠心肌损伤的保护作用，可能与促进心肌PPAR-γ表达有关。 [Objective]To investigate the mechanism of hydrogen sulfide delayed preconditioning on myocardial ischemia reperfusion injury in rats. [Methods]Three groups （ n = 10 animals/group） of Sprague Dawley male rats were studied： group S consisted of control rats that received no treatment, Group IR consisted of rats treated with NS （1.0 ml/kg iv） 24 h before ischemia, and Group H consisted of rats treated with NariS （0.05 mg/kg） 24 h be- fore ischemia. Group IR and H were subjected to ischemia by 30 rnin of coronary artery occlusion followed by 2 h of reperfusion. At the end of reperfusion, the plasma concentration of IL-6 and IL-10 were measured. The peroxi some proliferator-activated receptor γ （PPAR-γ） was measured by Western blotting. [ResultslCompared with Group S, Group H and Group IR had higher levels of IL-6 and IL-10 （ P 〈0.05）. Compared with Group IR, Group H had lower levels of IL-6 and higher levels of IL-10 （ P 〈0.05）. Compared with Group S, Group H and Group IR had lower PPAR-γ protein expression in myocardium （ P〈0.05）.Compared with Group IR, Group H had higher levels of PPAR-γ protein expression （ P〈0.05）.[Conclusion]The hydrogen sulfide delayed precondi- tioning attenuates myocardial IR injury possibly through up-regulating PPAR-γ expression in rats.

作者蒋海涛冉珂李志坚常业恬

机构地区岳阳市二医院麻醉科中南大学湘雅二医院麻醉科

出处《医学临床研究》 CAS 2016年第8期1484-1485,1488,共3页 Journal of Clinical Research

基金湖南省岳阳市科技局2012年第四批科技计划项目,湖南省科技厅项目（2013FJ4082）

关键词硫化氢/治疗应用缺血预处理心肌再灌注损伤/预防和控制过氧化物酶体增殖物激活受体 Hydrogen Sulfide/TU Ischemic Preconditioning Myocardial Reperfusion Injury/PC Peroxisome Proliferator-Activated Receptors

分类号 R542.2 [医药卫生—心血管疾病]

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