芥子气经腹腔和气管致大鼠急性肺损伤炎性反应的比较研究

A comparative study on inflammatory response due to sulfur mustard-induced acute lung injury in rat via the intraperitoneal and tracheal injection

目的经腹腔和气管建立大鼠芥子气(SM)肺损伤的动物模型,比较两种大鼠急性肺损伤模型炎性反应的差异。方法选取Sprague Dawley大鼠136只,分为5组,正常对照组8只,其他4个组(腹腔SM组、腹腔丙二醇对照组、气管SM组、气管丙二醇对照组)每组32只。腹腔SM组腹腔内注入稀释的SM 0.1mL(0.96LD50=8mg/kg),气管SM组气管内注入稀释的SM 0.1mL(0.98LD50=2mg/kg),正常对照组不做任何处理。ELISA法检测支气管肺泡灌洗液和血液标本,HE染色和免疫组织化学判断炎性反应情况。结果腹腔SM组各时间段支气管肺泡灌洗液蛋白含量和细胞计数与气管SM组相比显著升高(P<0.05);腹腔SM组各时间段血清TNF-α、IL-1β、IL-6与气管SM组相比显著升高(P<0.05);腹腔SM组各时间段肺泡间隔T、B淋巴细胞和巨噬细胞阳性表达率与气管SM组相比显著增加(P<0.05)。结论大鼠在SM LD50相似的情况下,腹腔SM组支气管肺泡灌洗液、肺泡间隔及血清炎性反应指标明显高于气管SM组。

Objective The purpose of this study was to establish animal model of sulfur mustard （SM）-induced acute lung injury in rats via .the intraperitoneal and the tracheal injection, in order to compare the difference of inflammatory reaction. Methods 136 male Sprague Dawley rats were selected,then were randomly divided into the five groups,the control group with 8 cases,other four groups （i. e. the intraperitoneal SM group, the intraperitoneal propylene glycol group, the tracheal SM group, the tracheal propylene glycol group） with 32 cases in each group. The intraperitoneal SM group .were injected intraperitoneally with di- luted SM 0.1 mL（0.96 LD50 ： 8 mg/kg）, the tracheal SM group were injected intratraeheally with diluted SM 0.1 mL（0. 98 LD50 = 2 mg/kg）,meanwhile the status quo was kept with the normal group. SM-induced inflammatory reaction was observed by bron- choalveolar lavage fluid （BALF）, serum examination. Hematoxylin Eosin staining, and immunohistochemical staining. Results Compared with the tracheal SM group at different time,protein contents and cell counts of BALF in the intraperitoneal SM group were significantly inceased,respectively （P〈0.05）. Compared with the tracheal SM group at different time, the levels of ser- um TNF-α, IL-1β, IL-6 in the intraperitoneal SM group were significantly inceased, respectively （P〈0.05）. The positive expression ratio of T lymphocytes, B lymphocytes and macrophages in intraperitoneal SM group at different time were increased compared with the tracheal SM group,respectively （P〈0.05）. Conclusion Under similar SM LD50 in rat,in the intraperitoneal SM group,inflam- matory reaction of BALF,alveolar septum,and serum were significantly higher than in the tracheal SM group.