ABCG2过表达对乳腺癌细胞上皮间质化能力的影响

Influence of ABCG2 overexpression on epithelial-mesenchymal transition of breast cancer cell

目的探讨三磷酸腺苷结合转运蛋白G超家族成员2(ABCG2)过表达对乳腺癌细胞上皮间质转化(EMT)能力的影响。方法构建ABCG2稳定过表达乳腺癌细胞株,检测ABCG2稳定过表达前后MCF-7乳腺癌细胞中E-cadherin蛋白和Ncadherin蛋白的表达情况。结果 E-cadherin蛋白表达在ABCG2过表达MCF-7乳腺癌细胞中明显低于常规MCF-7细胞(P<0.05),而N-cadherin蛋白表达在ABCG2过表达MCF-7乳腺癌细胞中明显高于常规MCF-7细胞(P<0.05)。结论 ABCG2过表达可以通过调控EMT相关蛋白的表达进而增强乳腺癌细胞的EMT能力,可能与乳腺癌的转移密切相关。

Objective To observe the influence on EMT of breast cancer cell caused by ABCG2 overexpression and to ap proach the mechanism about how ABCG2 to affect metastasis of breast cancer. Methods Stable ABCG2 overexpression breast cancer cell line was constructed. Western-blot was used for detection of expression of E-cadherin and N-cadherin before and after trans- fection to approach the mechanism. Results Expression of E-cadherin in ABCG2 overexpression MCF-7 breast cancer cell was much lower than normal MCF-7 breast cancer cell （P〈0. 05）. Expression of N-cadherin in ABCG2 overexpresslon MCF-7 breast cancer cell was much higher than normal MCF-7 breast cancer cell （P〈0.05）. Conclusion Overexpression of ABCG2 increases the expressions of EMT associated proteins of breast cancer cell so as to promote the EMT of breast cancer cell. ABCG2 might play an important role in promoting the metastasis of breast cancer.