芪红水煎液对免疫肝纤维化大鼠肝功能及肝纤维化标志物的影响

Effect of Astragalus Root Combined with Polygonum Orientale L. Decoction on Liver Function and Hepatic Fibrosis Index of Rats with Immunological Liver Fibrosis

目的观察芪红水煎液对猪血清诱导的免疫肝纤维化模型大鼠的肝功能及血清肝纤维化标志物的影响。方法模型制备采用腹腔注射无菌猪血清,每周2次,连续12周。成模后,用芪红水煎液干预治疗,秋水仙碱对照。实验结束血液生化检测肝功能,放射免疫法检测大鼠血清肝纤维化指标。结果与正常组大鼠比较,模型组大鼠血清ALT、AST、ALP水平均显著升高(均P<0.01),说明造模成功。秋水仙碱组ALT、AST、ALP水平与模型组大鼠相比较差别均不大(均P>0.05);而芪红水煎液组大鼠血清ALT、AST、ALP水平与模型组比较均改善(均P<0.01)。与正常组大鼠比较,模型组大鼠HA、LN、PCⅢ、IV-C明显升高(均P<0.01),说明造模成功。秋水仙碱组血清HA、LN、Ⅳ-C、PCⅢ与模型组比较,均有不同程度降低(P<0.01或P<0.05),Ⅳ-C则差别不大(P>0.05)。芪红水煎液组血清HA、LN、Ⅳ-C、PCⅢ也有不同程度降低(P<0.01或P<0.05)。结论提示芪红水煎液可以减轻肝细胞损伤,改善肝功能,保护肝组织,并降低肝纤维化程度,对肝纤维化有一定的防治作用。

Objective： To explore the influence of Astragalus root combined with Polygonum Orientalc L. decoc- tion on liver function and hepatic fibrosis index of rats with immunological liver fibrosis. Methods： A rat model of hepatic fibrosis was constructed by using abdominal injection with pig serum,and treated with Astragalus root combined with Polygonum Orientale L. decoction. Liver function was measured with automatic bio-ehemical ana- lyzer, and serum hepatic fibrosis index in rats was detected by radioimmunoassay. Results： ALT,AST, HA, LN,IV -C and PC-Ⅲ increased obviously in hepatic fibrosis rats （P 〈 0.05）. Serum liver fibrosis indexes in Astragalus root combined with Polygonum Orientale L. decoction decreased obviously （P 〈 0.05 or P 〈 0.01 ）. Conclusion： Astragalus root combined with Polygonum Orientale L. decoction haS definite prevention to immune hepatic fibro- sis ,and can reduce fibrosis degree;meanwhile,it can effectively decrease liver cell injury and improve liver func- tion.